3D printed nervous system on a chip. Issue 8 (16th December 2015)
- Record Type:
- Journal Article
- Title:
- 3D printed nervous system on a chip. Issue 8 (16th December 2015)
- Main Title:
- 3D printed nervous system on a chip
- Authors:
- Johnson, Blake N.
Lancaster, Karen Z.
Hogue, Ian B.
Meng, Fanben
Kong, Yong Lin
Enquist, Lynn W.
McAlpine, Michael C. - Abstract:
- Abstract : We introduce a bioinspired, customizable 3D printed nervous system on a chip for the study of nervous system viral infection. Abstract : Bioinspired organ-level in vitro platforms are emerging as effective technologies for fundamental research, drug discovery, and personalized healthcare. In particular, models for nervous system research are especially important, due to the complexity of neurological phenomena and challenges associated with developing targeted treatment of neurological disorders. Here we introduce an additive manufacturing-based approach in the form of a bioinspired, customizable 3D printed nervous system on a chip (3DNSC) for the study of viral infection in the nervous system. Micro-extrusion 3D printing strategies enabled the assembly of biomimetic scaffold components (microchannels and compartmented chambers) for the alignment of axonal networks and spatial organization of cellular components. Physiologically relevant studies of nervous system infection using the multiscale biomimetic device demonstrated the functionality of the in vitro platform. We found that Schwann cells participate in axon-to-cell viral spread but appear refractory to infection, exhibiting a multiplicity of infection (MOI) of 1.4 genomes per cell. These results suggest that 3D printing is a valuable approach for the prototyping of a customized model nervous system on a chip technology.
- Is Part Of:
- Lab on a chip. Volume 16:Issue 8(2016)
- Journal:
- Lab on a chip
- Issue:
- Volume 16:Issue 8(2016)
- Issue Display:
- Volume 16, Issue 8 (2016)
- Year:
- 2016
- Volume:
- 16
- Issue:
- 8
- Issue Sort Value:
- 2016-0016-0008-0000
- Page Start:
- 1393
- Page End:
- 1400
- Publication Date:
- 2015-12-16
- Subjects:
- Miniature electronic equipment -- Periodicals
Combinatorial chemistry -- Periodicals
Biotechnology -- Periodicals
543.0813 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/lc#!recentarticles&adv ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c5lc01270h ↗
- Languages:
- English
- ISSNs:
- 1473-0197
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 5137.730000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 752.xml