An efficient enzymatic production of N-acetyl-d-glucosamine from crude chitin powders. Issue 7 (9th December 2015)

• Record Type: Journal Article
• Authors: Zhang, Alei; Gao, Cong; Wang, Jing; Chen, Kequan; Ouyang, Pingkai
• Is Part Of: Green chemistry. Volume 18:Issue 7(2016)
• Page Start: 2147
• Page End: 2154
• Publication Date: 2015-12-09
• Subjects: Environmental chemistry -- Industrial applications -- Periodicals; Environmental management -- Periodicals; 660
• DOI: 10.1039/c5gc02242h
• Languages: English
• ISSNs: 1463-9262

Abstract

Abstract : In this study, we present an efficient affinity adsorption–enzymatic reaction integrated approach to produce N -acetyl-d -glucosamine (GlcNAc) from crude chitin powders (CP). Abstract : In this study, we present an efficient affinity adsorption–enzymatic reaction integrated approach to produce N -acetyl-d -glucosamine (GlcNAc) from crude chitin powders (CP). Chitinase was first purified from the crude enzyme mixture by affinity adsorption on the CP substrate, with ∼92.7% recovery of the enzyme achieved within 10 minutes in the presence of 20 g L −1 of CP under optimized conditions. The chitinase then efficiently converted 40 g L −1 of CP to 39.3 g L −1 of GlcNAc in a batch enzymatic reaction for 4 days, achieving nearly 100% conversion of the substrate CP. The product GlcNAc was easily separated from hydrolysates after destaining, concentrating, crystallizing and drying processes with a high purity of 98%. In addition, the chitinase can be conveniently reused for several batches by the affinity adsorption–enzymatic reaction approach, giving an average yield of 29.6 g L −1 of GlcNAc based on 30 g L −1 of CP as the substrate.