Tuning the inflammatory response to silver nanoparticles via quercetin in Caco-2 (co-)cultures as model of the human intestinal mucosa. (24th June 2016)

• Record Type: Journal Article
• Authors: Martirosyan, Alina; Grintzalis, Konstantinos; Polet, Madeleine; Laloux, Laurie; Schneider, Yves-Jacques
• Is Part Of: Toxicology letters. Volume 253(2016)
• Page Start: 36
• Page End: 45
• Publication Date: 2016-06-24
• Subjects: ACP acid phosphatase -- Ag-NPs silver nanoparticles -- ALP alkaline phosphatase -- AP apical -- BL basolateral -- CAT catalase -- DLS dynamic light scattering -- DMSO dimethylsulfoxide -- GIT gastrointestinal tract -- GR glutathione reductase -- GST glutathione-S-transferase -- HBSS Hank's Balanced Salt Solution -- HCS high content screening -- IL-6, 8 interleukin 6, 8 -- iNOS inducible-NOS -- MTT 3-(4, 5 dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide -- NBT nitroblue tetrazolium -- NO nitric oxide -- NOS nitric oxide synthase -- NR neutral red -- PBS phosphate buffered saline -- Q quercetin -- ROS reactive oxygen species -- RQ relative quantification -- SEM-EDX scanning electron microscopy with energy dispersive X-ray analysis -- t-BOOH t-butyl hydroperoxide -- TEM transmission electron microscopy; Silver nanoparticles -- Quercetin -- Gastrointestinal tract -- Inflammatory response -- Oxidative and nitrosative stress; Toxicology -- Periodicals; 363.179
• DOI: 10.1016/j.toxlet.2016.04.018
• Languages: English
• ISSNs: 0378-4274

Abstract

Highlights: Ag-NPs lead to inflammatory response in the intestine. Ag-NPs reduced Caco-2 cell viability, increased ROS/RNS and lipid peroxidation. Ag-NPs decreased secretion of the IL-8 protein and its gene expression. Silver ions had only partial contribution in the Ag-NP-induced toxicity. Quercetin protected the intestinal cells from the harmful effect of Ag-NPs. Abstract: Interaction of nanoparticles with food matrix components may cause unpredictable health complications. Using an improved Caco-2 cell-based in vitro (co-)culture model the potential of quercetin as one of the major food flavonoids to alter the effect of silver nanoparticles (Ag-NPs) <20 nm in the human intestinal mucosa at real life concentrations was investigated. Ag-NPs (15–90 μg/ml) decreased cell viability and reduced thiol groups, induced oxidative/nitrosative stress and lipid peroxidation and led to activity changes of various antioxidant enzymes after 3 h exposure. The contribution of Ag + ions within the concentrations released from nanoparticles was shown to be less important, compared to Ag-NPs. While leading to inflammatory response in the intestines, Ag-NPs, paradoxically, also showed a potential anti-infammatory effect manifested in down-regulated IL-8 levels. Quercetin, co-administered with Ag-NPs, led to a reduction of cytotoxicity, oxidative stress, and recovered metabolic activity of Caco-2 cells, suggesting the protective effects of this flavonoid against the harmful effect of Ag-NPs. Quercetin not only alleviated the effect of Ag-NPs on the gastrointestinal cells, but also demonstrated a potential to serve as a tool for reversible modulation of intestinal permeability.