Electrochemical detection of protein glycosylation using lectin and protein–gold affinity interactions. Issue 8 (16th March 2016)

• Record Type: Journal Article
• Authors: Yadav, Sharda; Carrascosa, Laura G.; Sina, Abu A. I.; Shiddiky, Muhammad J. A.; Hill, Michelle M.; Trau, Matt
• Is Part Of: Analyst. Volume 141:Issue 8(2016)
• Page Start: 2356
• Page End: 2361
• Publication Date: 2016-03-16
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c6an00528d
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : New electrochemical method to detect glycosylation of protein using lectin and protein gold interaction. Abstract : We report a new method for the electrochemical detection of glycosylation on proteins, which relies on lectin–protein interaction on a bare gold electrode. The target protein isolated by immunoaffinity is directly adsorbed onto a gold surface and its glycosylation status is retrieved by subsequent addition of specific lectins. The adsorption and subsequent recognition process is monitored electrochemically in the presence of [Fe(CN)6 ] 3−/4− redox system. By decoupling target protein capture from glycosylation read-out steps, this approach circumvents unwanted antibody–lectin crosstalk while enabling specific glycosylation detection of a glycoprotein in serum-spiked samples in less than 1 h.