Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer. Issue 10 (3rd February 2016)

• Record Type: Journal Article
• Authors: Fu, Guanglei; Sanjay, Sharma T.; Dou, Maowei; Li, XiuJun
• Is Part Of: Nanoscale. Volume 8:Issue 10(2016)
• Page Start: 5422
• Page End: 5427
• Publication Date: 2016-02-03
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c5nr09051b
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Based on the nanoparticle-mediated photothermal effect, a new photothermal biomolecular quantitation method using a common thermometer as the signal reader was developed. Abstract : A new biomolecular quantitation method, nanoparticle-mediated photothermal bioassay, using a common thermometer as the signal reader was developed. Using an immunoassay as a proof of concept, iron oxide nanoparticles (NPs) captured in the sandwich-type assay system were transformed into a near-infrared (NIR) laser-driven photothermal agent, Prussian blue (PB) NPs, which acted as a photothermal probe to convert the assay signal into heat through the photothermal effect, thus allowing sensitive biomolecular quantitation using a thermometer. This is the first report of biomolecular quantitation using a thermometer and also serves as the first attempt to introduce the nanoparticle-mediated photothermal effect for bioassays.