Simultaneous determination of water‐soluble whitening ingredients and adenosine in different cosmetic formulations by high‐performance liquid chromatography coupled with photodiode array detection. (23rd December 2015)

• Record Type: Journal Article
• Authors: Jeon, J.‐S.; Kim, H.‐T.; Kim, M.‐G.; Oh, M.‐S.; Hong, S.‐R.; Yoon, M.‐H.; Cho, S.‐M.; Shin, H.‐C.; Shim, J.‐H.; Ramadan, A.; Abd El‐Aty, A. M.
• Is Part Of: International journal of cosmetic science. Volume 38:Number 3(2016)
• Page Start: 286
• Page End: 293
• Publication Date: 2015-12-23
• Subjects: adenosine -- arbutin -- ascorbyl glucoside -- ethyl ascorbyl ether -- high‐performance liquid chromatography -- niacinamide; Cosmetics -- Periodicals; 668.5505
• DOI: 10.1111/ics.12292
• Languages: English
• ISSNs: 0142-5463

Abstract

Abstract: Objective: The Korean Cosmetic Act regulates the use of functional cosmetics) by the law. Four functional cosmetic groups, whitening, anti‐wrinkle, UV protection and combination of whitening and anti‐wrinkle, were categorized according to the Korean Cosmetic Act and Functional Cosmetics Codex. In this study, high‐performance liquid chromatography (HPLC) coupled with photodiode array detection (DAD) was employed for the simultaneous detection of arbutin (and its decomposition product, hydroquinone), niacinamide, ascorbyl glucoside, ethyl ascorbyl ether and adenosine in functional cosmetic products such as creams, emulsions and lotions. Methods: Separation by HPLC‐DAD was conducted using a C18 column with a gradient elution of 5 mm KH2PO4 buffer (containing 0.1% phosphoric acid) and methanol (containing 0.1% phosphoric acid). The wavelengths for the detection of arbutin, hydroquinone, niacinamide, adenosine, ascorbyl glucoside and ethyl ascorbyl ether were 283, 289, 261, 257, 238 and 245 nm, respectively. Results: This method exhibited good linearity ( R 2 ≥ 0.999), precision (expressed as relative standard deviation (RSD) < 2%) and mean recoveries (89.42–104.89%). The results obtained by monitoring 100 market samples showed that the detected levels of the tested materials are within the acceptable authorized concentration. Conclusion: The method developed herein is simple and can be used for market survey and quality control of functional cosmetics. Abstract : Arbutin (and its decomposed product, hydroquinone), niacinamide, ascorbyl glucoside, ethyl ascorbyl ether, and adenosine were determined simultaneously in functional cosmetic products (cream, emulsion, and lotion) using HPLC‐DAD. (A) Standard solutions: niacinamide (200.8 μ g mL −1, 261 nm), ascorbyl glucoside (200.1 μ g mL −1, 238 nm), arbutin (200.5 μ g mL −1, 283 nm), adenosine (4.2 μ g mL −1, 257 nm) hydroquinone (20.1 μ g mL −1, 289 nm), and ethyl ascorbyl ether (100.5 μ g mL −1, 245 nm), (B & c) cream sample solution, (D) emulsion sample solution, and (E) lotion sample solution.