A multipurpose biochip for food pathogen detection. Issue 15 (17th March 2016)

• Record Type: Journal Article
• Authors: Primiceri, Elisabetta; Chiriacò, Maria Serena; de Feo, Francesco; Santovito, Elisa; Fusco, Vincenzina; Maruccio, Giuseppe
• Is Part Of: Analytical methods. Volume 8:Issue 15(2016)
• Page Start: 3055
• Page End: 3060
• Publication Date: 2016-03-17
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c5ay03295d
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : We realized an innovative biosensing platform with high sensitivity, low-cost and label-free features for S. aureus and L. monocytogenes detection from meat. Abstract : Foodborne illnesses caused by the ingestion of foods contaminated with pathogens and/or their toxins are still one of the major public health threats worldwide. Disposable devices, allowing the on-site, early and multiplexed quantitative detection of pathogenic bacteria are therefore highly sought. Herein, we report biochips that are able to quantitatively detect two of the most common food-associated pathogens, namely Listeria monocytogenes and Staphylococcus aureus from the suspensions of bacteria stationary-phase broth culture. With a detection limit as low as 5.00 CFU ml −1 for L. monocytogenes and 1.26 CFU ml −1 for S. aureus, our platform may be a promising point-of-care device not only for clinical and food diagnostics but also for biosecurity purposes.