Fluorescent Bead–DNA Conjugate‐based Dual Signal Amplification Technology. (21st April 2016)

• Record Type: Journal Article
• Authors: Sonawane, Mukesh Digambar; Lee, Hae Ryong; Nimse, Satish Balasaheb; Song, Keum‐Soo; Kim, Taisun; Kim, Sang Cheol
• Is Part Of: Bulletin of the Korean Chemical Society. Volume 37:Number 5(2016)
• Page Start: 655
• Page End: 659
• Publication Date: 2016-04-21
• Subjects: Microarray -- Fluosphere -- Fluoro‐microbeads -- Signal amplification -- 9G DNA chips; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/bkcs.10744
• Languages: English
• ISSNs: 0253-2964

Abstract

Abstract : It is the first report of the development of a dual signal amplification technology for DNA microarrays by synthesizing and applying the FB–DNA conjugates. Abstract : One of the major challenges in DNA microarrays for their widespread application is assay sensitivity. Therefore, to achieve higher sensitivity, we developed a dual signal amplification technology based on the fluorescence beads (FB). It is the first report of the development of a dual signal amplification technology for DNA microarrays by synthesizing and applying the FB–DNA conjugates. The FB–DNA conjugates and Cy5‐T1‐FB‐mixed‐DNA demonstrated ~250–325‐fold increase in sensitivity when compared with the conventional use of Cy5‐labeled oligonucleotides such as Cy5‐T1. Furthermore, the Cy5‐T1‐FB‐mixed‐DNA demonstrated ~5–1.3‐fold increase in the sensitivity when compared with that of the FB–DNA conjugates alone.