Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway. (May 2016)

• Record Type: Journal Article
• Authors: Ge, Zhenzhen; Zhu, Wei; Peng, Jinming; Deng, Xiangyi; Li, Chunmei
• Is Part Of: Journal of functional foods. Volume 23(2016)
• Page Start: 283
• Page End: 293
• Publication Date: 2016-05
• Subjects: Persimmon tannin -- Cholesterol homeostasis -- LXRα -- ABCA1 -- CYP7A1; LXRα Liver X receptor α -- NPC1L1 Niemann-Pick C1-Like 1 -- 22-NBD-cholesterol 22-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino)-23, 24-bisnr-5-cholen-3β-ol -- DP degree of polymerisation -- siRNA small interfering RNA -- SREBP-2 sterols regulatory element-binding protein 2 -- RCT reverse cholesterol transport -- ABCA1 ATP-binding cassette transporter A1 -- SR-BI scavenger receptor class B type I -- CYP7A1 cholesterol-7α-hydroxylase -- HDL high-density lipoprotein -- ApoAI apolipoproteins AI -- ApoB apolipoproteins B -- PPARγ peroxisome proliferator-activated receptor γ; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.02.033
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Persimmon tannin shows significant hypocholesterolaemic properties in in vitro studies. The uptake of 22-NBD-cholesterol can be inhibited by persimmon tannin intervention. Persimmon tannin down-regulated the expression of NPC1L1 genes. Persimmon tannin exhibited potential in promoting cellular cholesterol efflux. The regulation of persimmon tannin on cholesterol homeostasis was LXRα independent. Abstract: Persimmon tannin (proanthocyanidins) has been proven to exert significant hypocholesterolaemic effects in a variety of models. However, the regulatory mechanisms of persimmon tannin on cholesterol metabolism have yet to be fully understood. The present study investigated the regulation of persimmon tannin on the expression of genes critical for cholesterol absorption and cholesterol efflux. Our results indicated that persimmon tannin reduced the uptake of 22-NBD-cholesterol and inhibited the accumulation of cholesterol significantly in HepG2 and Caco-2 cell lines. The mRNA and proteins were quantified using real time PCR and western blot, respectively. The expression of SREBP-2 gene, critical for cholesterol synthesis, and NPC1L1 involved in cholesterol absorption were significantly inhibited by persimmon tannin. Moreover, persimmon tannin showed potential in promoting cholesterol efflux through up-regulating mRNA and/or protein levels of ABCA1, ABCG1, SR-BI, CYP7A1 and ABCG5/G8. However, the regulation effects of persimmon tannin on cholesterol homeostasis in both cell lines were LXRα independent.