Degradation of poly(butylene succinate) and poly(butylene succinate-co-butylene adipate) by a lipase from yeast Cryptococcus sp. grown on agro-industrial residues. (May 2016)

• Record Type: Journal Article
• Authors: Thirunavukarasu, Kathirvel; Purushothaman, Subramanian; Sridevi, Janardhanam; Aarthy, Mayilvahanan; Gowthaman, Marichetti Kuppuswami; Nakajima-Kambe, Toshiaki; Kamini, Numbi Ramudu
• Is Part Of: International biodeterioration & biodegradation. Volume 110(2016)
• Page Start: 99
• Page End: 107
• Publication Date: 2016-05
• Subjects: Cryptococcus sp. -- Lipase -- Cottonseed oil cake -- Poly(butylene succinate) -- Poly(butylene succinate-co-butylene adipate) -- Degradation; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2016.03.005
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: The yeast, Cryptococcus sp. MTCC 5455 was grown on various agro-industrial residues for the production of lipase. A maximum lipase activity of 753 ± 19 U g dry substrate −1 (U gds −1 ) and a biomass of 103 ± 5 mg gds −1 was obtained at 25 °C and 120 h using cottonseed oil cake with 71% moisture content and 30% (v/w) inoculum. The recovery of the enzyme was increased by 14.1%, when the fermented substrate was extracted in 2 mM CaCl2 .2H2 O solution. The crude enzyme partially purified by (NH4 )2 SO4 precipitation showed a major 22 kDa protein on SDS-PAGE. The enzyme has good potential for hydrolysis of poly(butylene succinate) (PBS) and poly(butylene succinate-co-butylene adipate) (PBSA) and complete degradation of the polymeric films were observed at 72 h and 16 h respectively. The degradation was evaluated by Fourier transform infrared (FTIR) and 1 H NMR spectroscopy. The complete hydrolysis of polymers by Cryptococcus sp. lipase makes the process ideal and also serves as a baseline for its exploitation in polymer degradation. Highlights: Cryptococcus sp. lipase from agro residues was used for PBS and PBSA degradation. Growth of yeast on SSF was investigated by scanning electron microscopy. Degradation of PBS and PBSA was complete at 72 and 16 h respectively. FTIR and NMR studies confirmed the degradation by cleavage of ester bonds.