Activation of immobilized Clostridium saccharoperbutylacetonicum N1-4 for butanol production under different oscillatory frequencies and chemical buffers. (May 2016)

• Record Type: Journal Article
• Authors: Chen, Wen-Hsing; Chen, Yu-Chen; Chaiprapat, Sumate
• Is Part Of: International biodeterioration & biodegradation. Volume 110(2016)
• Page Start: 129
• Page End: 135
• Publication Date: 2016-05
• Subjects: Butanol -- Activation -- Immobilization -- Fermentation -- PVA-sodium alginate -- Clostridium; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2016.03.014
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: This study aimed to investigate the activation of Clostridium saccharoperbutylacetonicum N1-4 immobilized by polyvinyl alcohol (PVA)-sodium alginate to improve butanol production under different oscillatory frequencies and chemical buffers. The results show butanol yields increased from 0.24 to 0.28 g g −1 sucrose consumed by un-subcultured immobilized cells to the level of 0.28–0.35 g g −1 sucrose consumed by subcultured immobilized cells, and there was no discrepancy with the yield of 0.28–0.31 g g −1 sucrose consumed by the free cells. The butanol production rates also increased 5- to 12-fold of those of the un-subcultured immobilized cells when the oscillatory frequencies were controlled from 0 to 3.4 Hz. In addition, an oscillating frequency of 3.4 Hz not only caused a reduction of the free cell concentration in butanol fermentation but also decreased the butanol yield when subcultured immobilized cells were employed. For the effect of the chemical buffers, a butanol production rate of 5243 mg l −1 d −1 for acetate buffer was 3-fold that of citrate. However, the butanol yields of acetate and citrate were not significantly different. The activation via subculture method improves the performance of butanol production by PVA-sodium alginate immobilized Clostridia . Acetate is a preferable buffer chemical for biobutanol production. Highlights: Subculture enhances butanol production by immobilized solvent-producing clostridia. Using acetate as a buffer solution is superior to citrate for ABE fermentation. High oscillating frequency breaks down the matrix of PVA-sodium alginate. High oscillating frequency reduces the concentration of solvent-producing clostridia.