Defatted bovine milk fat globule membrane inhibits association of enterohaemorrhagic Escherichia coli O157:H7 with human HT-29 cells. (August 2016)

• Record Type: Journal Article
• Authors: Ross, Sarah A.; Lane, Jonathan A.; Kilcoyne, Michelle; Joshi, Lokesh; Hickey, Rita M.
• Is Part Of: International dairy journal. Volume 59(2016:Aug.)
• Page Start: 36
• Page End: 43
• Publication Date: 2016-08
• Subjects: Dairying -- Periodicals; Industrie laitière -- Périodiques; Electronic journals; 338.1762142
• DOI: 10.1016/j.idairyj.2016.03.001
• Languages: English
• ISSNs: 0958-6946

Abstract

Abstract: The bovine milk fat globule membrane (MFGM) is a source of food-derived glycans that can offer an approach to prevent Escherichia coli O157:H7 infection by inhibiting attachment of the pathogen to host cells. Such glycans may decrease the need for antibiotic treatment by acting as prophylactics. In this study, we generated a defatted bovine MFGM fraction, rich in proteins and glycoproteins, and demonstrated its ability to prevent the association of several enterohaemorrhagic E. coli O157:H7 strains with human colonic adenocarcinoma, HT-29 cells. This defatted MFGM fraction reduced bacterial association with HT-29 cells in a concentration dependent and strain specific manner. This study may present a new approach to mitigate the adverse health effects caused by E. coli infections in humans.