MiR-1934, downregulated in obesity, protects against low-grade inflammation in adipocytes. (15th June 2016)

• Record Type: Journal Article
• Authors: Liu, Lulu; Li, Qifu; Xiao, Xiaoqiu; Wu, Chaodong; Gao, Rufei; Peng, Chuan; Li, Danting; Zhang, Wenlong; Du, Tingting; Wang, Yue; Yang, Shumin; Zhen, Qianna; Ge, Qian
• Is Part Of: Molecular and cellular endocrinology. Volume 428(2016)
• Page Start: 109
• Page End: 117
• Publication Date: 2016-06-15
• Subjects: Obesity -- Low-grade inflammation -- microRNA -- Adipose tissue -- Adipocyte; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2016.03.026
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: It has been previously demonstrated that miR-1934 was specially regulated by adiponectin, an anti-inflammatory adipokine, in adipose tissue (AT) in vivo . Herein we investigated the role of miR1934 in the regulation of inflammatory response. Compared with chow-diet fed mice, miR1934 expression was down-regulated in epididymal AT of high-fat diet-induced obese mice. miR1934 expression was down-regulated as well in omental AT of obese subjects in comparison with lean subjects. The circulating miR-1934 was also lower in obese subjects and its levels were correlated negatively with body mass index (BMI), waist circumference (WC), low-density lipoprotein cholesterol (LDL-c), insulin resistance and high-sensitivity C-reactive protein (hs-CRP). The down-regulation of miR1934 in obesity was mimicked by TNF-α treatment of 3T3-L1 adipocytes. Moreover, overexpression of miR1934 suppressed the TNF-α-induced gene expression of interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in 3T3-L1 adipocytes. De novo formed AT in nude mice transplanted with 3T3-L1 preadipocytes overexpressing miR1934 displayed a reduction in IL-6, TNF-α, IL-1β and an enhancement in IL-10 gene expression when compared with transplant with 3T3-L1 preadipocytes overexpressing miR1934 scrambled control sequence. These results suggest that miR1934 is an important anti-inflammatory factor and may represent a novel mechanism for controlling AT inflammation. Highlights: miR-1934 was firstly found to be downregulated in mice and human obesity. miR-1934 was secreted in circulation. The circulating miR-1934 correlated with obesity and obesity-related inflammation. The anti-inflammatory effect of miR-1934 in adipocytes was validated by both in vitro and in vivo experiments.