The effect of gallic acid on cytotoxicity, Ca2+ homeostasis and ROS production in DBTRG-05MG human glioblastoma cells and CTX TNA2 rat astrocytes. (25th May 2016)

• Record Type: Journal Article
• Authors: Hsu, Shu-Shong; Chou, Chiang-Ting; Liao, Wei-Chuan; Shieh, Pochuen; Kuo, Daih-Huang; Kuo, Chun-Chi; Jan, Chung-Ren; Liang, Wei-Zhe
• Is Part Of: Chemico-biological interactions. Volume 252(2016)
• Page Start: 61
• Page End: 73
• Publication Date: 2016-05-25
• Subjects: Ca2+ -- Cytotoxicity -- Gallic acid -- Human glioblastoma cells -- ROS -- Mitochondrial apoptotic pathway; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2016.04.010
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Gallic acid, a polyhydroxylphenolic compound, is widely distributed in various plants, fruits and foods. It has been shown that gallic acid passes into blood brain barrier and reaches the brain tissue of middle cerebral artery occlusion rats. However, the effect of gallic acid on Ca 2+ signaling in glia cells is unknown. This study explored whether gallic acid affected Ca 2+ homeostasis and induced Ca 2+ -associated cytotoxicity in DBTRG-05MG human glioblastoma cells and CTX TNA2 rat astrocytes. Gallic acid (20–40 μM) concentration-dependently induced cytotoxicity and intracellular Ca 2+ level ([Ca 2+ ]i ) increases in DBTRG-05MG cells but not in CTX TNA2 cells. In DBTRG-05MG cells, the Ca 2+ response was decreased by half by removal of extracellular Ca 2+ . In Ca 2+ -containing medium, gallic acid-induced Ca 2+ entry was inhibited by store-operated Ca 2+ channel inhibitors (2-APB, econazole and SKF96365). In Ca 2+ -free medium, pretreatment with the endoplasmic reticulum Ca 2+ pump inhibitor thapsigargin abolished gallic acid-induced [Ca 2+ ]i increases. Conversely, incubation with gallic acid also abolished thapsigargin-induced [Ca 2+ ]i increases. Inhibition of phospholipase C with U73122 abolished gallic acid-induced [Ca 2+ ]i increases. Gallic acid significantly caused cytotoxicity in DBTRG-05MG cells, which was partially prevented by prechelating cytosolic Ca 2+ with BAPTA-AM. Moreover, gallic acid activated mitochondrial apoptotic pathways that involved ROS production. Together, in DBTRG-05MG cells but not in CTX TNA2 cells, gallic acid induced [Ca 2+ ]i increases by causing Ca 2+ entry via 2-APB, econazole and SKF96365-sensitive store-operated Ca 2+ entry, and phospholipase C-dependent release from the endoplasmic reticulum. This Ca 2+ signal subsequently evoked mitochondrial pathways of apoptosis that involved ROS production. Graphical abstract: Highlights: The effects of gallic acid on DBTRG-05MG cells and CTX TNA2 cells were explored. In DBTRG-05MG cells, gallic acid evoked Ca 2+ movement including Ca 2+ release and Ca 2+ entry. In DBTRG-05MG cells, gallic acid activated Ca 2+ -associated mitochondrial pathways of apoptosis that involved ROS production.