Nasunin, a new player in the field of osteoblast protection against oxidative stress. (May 2016)

• Record Type: Journal Article
• Authors: Casati, Lavinia; Pagani, Francesca; Braga, Pier Carlo; Lo Scalzo, Roberto; Sibilia, Valeria
• Is Part Of: Journal of functional foods. Volume 23(2016)
• Page Start: 474
• Page End: 484
• Publication Date: 2016-05
• Subjects: Nasunin -- Osteoblasts -- Oxidative stress -- Osteoblastic dysfunction -- Phosphoinositide 3-kinase/AKT pathway; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.03.007
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Oxidative stress is involved in the age-related decrease in osteoblast activity. Antioxidants are known to protect against oxidative damage during ageing. Nasunin is a natural antioxidant from the eggplant peel. Nasunin prevents MC3T3-E1 osteoblast cells from oxidative damage. The protective effect of nasunin involves PI3K/Akt pathway. Abstract: This study investigated whether nasunin, the major component of anthocyanin pigment of eggplant ( Solanum melongena L.), known for its antioxidant effects, counteracts tert- butyl hydroperoxide ( t -BHP)-induced oxidative damage in MC3T3-E1 osteoblastic cells. Pretreatment with purified nasunin (10 −10 –10 −6 M) significantly increased viability and reduced apoptosis of MC3T3-E1 cells cultured with t -BHP (250 µM, 3 h). Furthermore, nasunin (10 −9 M) prevented t -BHP-induced osteoblastic dysfunction and changes in the cytoskeleton organisation due to both an increase of intracellular glutathione and a decrease of intracellular reactive oxygen species. Moreover, t -BHP-induced reduction in osteoblast differentiation markers, such as alkaline phosphatase and collagen content, was recovered in nasunin-pretreated cells. Nasunin protects against t -BHP-induced osteoblastic dysfunction by activating the phosphoinositide 3-kinase/Akt signalling pathway (PI3K/Akt) since LY294002, a PI3K-specific inhibitor, worsened the cytotoxic effect of t -BHP and reversed the protective action of nasunin. Furthermore, nasunin prevented the decreased Akt phosphorylation observed in t -BHP-treated cells.