A 'turn‐on' fluorescent chemosensor for quantification of serum albumin in aqueous solution at neutral pH. (10th November 2015)

• Record Type: Journal Article
• Authors: Li, Yuanyuan; Li, Kai; He, Juan
• Is Part Of: Luminescence. Volume 31:Number 3(2016:May/Jun.)
• Page Start: 905
• Page End: 910
• Publication Date: 2015-11-10
• Subjects: serum albumin -- fluorescence turn‐on -- chemosensor -- long‐wavelength emission; Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.3052
• Languages: English
• ISSNs: 1522-7235

Abstract

Abstract: A fluorescent chemosensor 1 (4‐diethylamino‐2′‐hydroxychalcone) for detecting serum albumin with long‐wavelength emission, good selectivity and facile synthesis was reported. Upon the addition of bovine serum albumin (BSA) to an aqueous solution of 1 at neutral pH, a 'turn‐on' fluorescence response was observed at 596 nm based on a hydrophobic binding mode between 1 and BSA. A linear range of 0.10–1.00 mg/mL and a detection limit of 9.1 µg/mL for BSA were obtained, respectively. Moreover, 1 was successfully applied to detect BSA in real bovine serum samples with satisfied recovery and accuracy, which suggested that 1 could serve as a valid and effective fluorescent chemosensor for quantification of BSA. Copyright © 2015 John Wiley & Sons, Ltd.