Microvesicle-associated tissue factor procoagulant activity for the preoperative diagnosis of ovarian cancer. Issue 141 (May 2016)

• Record Type: Journal Article
• Authors: Claussen, Carlota; Rausch, Alma-Verena; Lezius, Susanne; Amirkhosravi, Ali; Davila, Monica; Francis, John L.; Hisada, Yohei M.; Mackman, Nigel; Bokemeyer, Carsten; Schmalfeldt, Barbara; Mahner, Sven; Langer, Florian
• Is Part Of: Thrombosis research. Issue 141(2016)
• Page Start: 39
• Page End: 48
• Publication Date: 2016-05
• Subjects: Tissue factor -- Ovarian cancer -- Microvesicles -- D-dimer -- Venous thromboembolism; Thrombosis -- Periodicals; 616.135
• DOI: 10.1016/j.thromres.2016.03.002
• Languages: English
• ISSNs: 0049-3848

Abstract

Abstract: Background: Tissue factor (TF) is involved in tumor growth and metastasis and contributes to venous thromboembolism (VTE) in cancer, including gynecological malignancies. The diagnostic value of microvesicle-associated TF procoagulant activity (MV TF PCA) in women with suspected ovarian cancer, however, has not been studied. Objective: To evaluate MV TF PCA as a diagnostic tool in women with an ovarian mass of unknown etiology and as a predictive biomarker for perioperative VTE. Methods: Plasma MVs were isolated by high-speed centrifugation and analyzed for TF-specific PCA by single-stage clotting assay. In addition, plasma TF antigen and soluble P-selectin (sCD62P) were measured by ELISA. Results: D-Dimer, MV TF PCA, and sCD62P, but not the tumor marker, CA-125, significantly differentiated patients with malignant ( n = 40) from those with benign tumors ( n = 15) and healthy controls ( n = 34). In cancer patients, only D-Dimer and CA-125 correlated with the FIGO stage. An abnormal D-dimer had the highest sensitivity for the diagnosis of cancer, while MV TF PCA above the ROC curve-derived cut-off value of 182 U/mL had the highest specificity. By multivariate logistic regression analysis, addition of MV TF PCA conferred diagnostic benefit to the single variables, CA-125 ( p = 0.052) and D-dimer ( p = 0.019). Perioperative VTE occurred in 16% of cancer patients and was associated with an advanced FIGO stage, but not MV TF PCA. There was no difference in plasma TF antigen levels between study groups. Conclusions: MV TF PCA, but not plasma TF antigen, may provide valuable additional information for the diagnostic work-up of women with suspected ovarian cancer. Highlights: Preoperative levels of MV TF PCA are increased in patients with ovarian cancer. MV TF PCA is not correlated with the tumor stage. Addition of MV TF PCA confers diagnostic benefit to D-dimer and CA-125. Assessment of MV TF PCA cannot be replaced by quantification of plasma TF antigen.