A novel stoichiometries methodology to quantify functional microorganisms in simultaneous (partial) nitrification-endogenous denitrification and phosphorus removal (SNEDPR). (15th May 2016)

• Record Type: Journal Article
• Authors: Wang, Xiaoxia; Wang, Shuying; Zhao, Ji; Dai, Xian; Li, Baikun; Peng, Yongzhen
• Is Part Of: Water research. Volume 95(2016)
• Page Start: 319
• Page End: 329
• Publication Date: 2016-05-15
• Subjects: Simultaneous nitrification-endogenous denitrification and phosphorus removal (SNEDPR) -- Partial nitrification (PN) -- Phosphorous accumulating organisms (PAOs) -- Denitrifying glycogen accumulating organisms (DGAOs) -- Stoichiometric model; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2015.12.046
• Languages: English
• ISSNs: 0043-1354

Abstract

Abstract: Although efficient removal of carbon (C), nitrogen (N) and phosphorous (P) from wastewater with low C/N ratio was achieved in anaerobic/aerobic simultaneous nitrification-endogenous denitrification and phosphorus removal (SNEDPR) systems, the removal pathways and metabolic transformations in this complex system are unclear. This work targeted at developing the stoichiometric models for denitrifying glycogen organisms (DGAOs) via nitrite and nitrate (DGAONi and DGAONa ), and demonstrating a novel methodology to quantify diverse functional microorganisms (e.g. ammonia and nitrite oxidizing bacteria, aerobic phosphorus accumulating organisms (APAOs), denitrifying PAOs (DPAOs) and aerobic GAOs (AGAOs)) for the removal of C, N and P. The results showed that the anaerobic intracellular carbon storage (CODintra ) was mainly accomplished by GAOs, and PAOs were only responsible for about 40% of CODintra through a stable P release. At the aerobic stage, 84.9% of P was removed by APAOs with 15.1% left by DPAOs, while 64.6% of N was removed by DGAOs (45.8% by DGAONi and 18.8% by DGAONa ) with 18.1% by DPAOs and 17.3% by bacterial growth. High proportion of N removal via nitrite (partial nitrification-endogenous denitrification) (71%) saved 7.3% aeration and 38% intracellular carbon demand. However, AGAOs still activated well at the aerobic intercellular carbon consumption, which limited the further improvement of N removal efficiency. By elucidating the nutrient removal pathways among diverse functional microorganisms, the methodology developed in this study could accelerate the nutrient removal in the SNEDPR process. Graphical abstract: Highlights: A novel methodology was developed to quantify functional microorganisms in SNEDPR. The first stoichiometric model of DGAOs via nitrite and nitrate was developed. DGAOs conducted 64.6% of aerobic N removal and APAOs conducted 84.9% of P uptake. N removal is improved by reducing AGAO activity and enhancing DGAO activity. PNED reduced 7.3% aeration and 38% intracellular carbon demand.