Recombinant Mycobacterium bovis bacillus Calmette–Guérin expressing Ag85B-IL-7 fusion protein enhances IL-17A-producing innate γδ T cells. Issue 22 (11th May 2016)

• Record Type: Journal Article
• Authors: Hatano, Shinya; Tamura, Toshiki; Umemura, Masayuki; Matsuzaki, Goro; Ohara, Naoya; Yoshikai, Yasunobu
• Is Part Of: Vaccine. Volume 34:Issue 22(2016)
• Page Start: 2490
• Page End: 2495
• Publication Date: 2016-05-11
• Subjects: Ag antigen -- BCG Mycobacterium bovis bacillus Calmette–Guérin -- PPD purified protein derivative of tuberculin -- MNC mononuclear cells -- TCR T cell receptor -- IL interleukin -- Tg transgenic -- r recombinant -- PMA phorbol 12-myristate 13-acetate -- mAb monoclonal antibody -- R receptor -- IFN-γ interferon γ; γδ T cells -- Cytokines -- IL-7 -- IL-17A -- BCG -- Bacteria; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2016.03.096
• Languages: English
• ISSNs: 0264-410X

Abstract

Highlights: A recombinant BCG expressing Ag85B-IL-7 fusion protein was constructed. IL-17A+ γδ T and Th1 cells were increased in mice inoculated with rBCG-Ag85B-IL-7. Th1 cells were impaired in rBCG inoculated mice lacking IL-17A+ γδ T cells. IL-17A+ γδ T cells subsequently augment the Th1 response to BCG. Abstract: Interleukin 7 (IL-7) has an important function in the development and maintenance of IL-17A+ γδ T cells. We here constructed a recombinant Mycobacterium bovis bacillus Calmette–Guérin expressing antigen 85B (Ag85B)-IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG-Ag85B-IL-7. rBCG-Ag85B-IL-7 was the same in number as control rBCG expressing Ag85B (rBCG-Ag85B) in the lung at the early stage after intravenous inoculation, whereas the numbers of IL-17A+ γδ T cells and Ag-specific Th1 cells were significantly higher in the lungs of mice inoculated with rBCG-Ag85B-IL-7 than those inoculated with rBCG-Ag85B. The Ag-specific Th1 cell response was impaired in mice lacking IL-17A+ γδ T cells after inoculation with rBCG-Ag85B-IL-7. Thus, rBCG-Ag85B-IL-7 increases the pool size of IL-17A+ γδ T cells, which subsequently augment the Th1 response to mycobacterial infection.