Antibiosis levels of common bean genotypes toward Zabrotes subfasciatus (Boheman) (Coleoptera: Bruchidae) and its correlation with flavonoids. (May 2016)

• Record Type: Journal Article
• Authors: Eduardo, Wellington Ivo; Boiça Júnior, Arlindo Leal; Moraes, Renato Franco Oliveira de; Chiorato, Alisson Fernando; Perlatti, Bruno; Forim, Moacir Rossi
• Is Part Of: Journal of stored products research. Volume 67(2016)
• Page Start: 63
• Page End: 70
• Publication Date: 2016-05
• Subjects: Phaseolus vulgaris -- Host plant resistance to insect -- Multivariate analysis -- Secondary metabolites -- Bean weevil; Food -- Storage -- Periodicals; Farm produce -- Storage -- Diseases and injuries -- Periodicals; Entomology -- Periodicals; Food Contamination -- Periodicals; Food Preservation -- Periodicals; Insect Control -- Periodicals; Aliments -- Entreposage -- Périodiques; Produits agricoles -- Entreposage -- Maladies et dommages -- Périodiques; Electronic journals; 631.568
• DOI: 10.1016/j.jspr.2016.01.006
• Languages: English
• ISSNs: 0022-474X

Abstract

Abstract: Expression of the antibiosis-resistance category to weevils has been evaluated in several bean genotypes with very promising results. Among the several causes responsible for this resistance category the arcelin protein and trypsin inhibitors stand out. Other mechanisms may be associated with plant resistance to the attack of weevils; however, few studies seek to discover these possible causes. Thus, our research aimed at identifying bean genotypes resistant to Zabrotes subfasciatus, classify them into resistance levels, quantify the content of flavonoids, and correlate it with data obtained from the genotypes. An antibiosis test was performed with beans of 43 genotypes and 40 replications (bean grains) under a completely randomized design. The biological parameters recorded from Z. subfasciatus were the periods from egg to larvae, larvae to adult, egg to adult, longevity and life cycle, in addition to egg viability, adults emerged, susceptibility relative index, adult weight, sex ratio, and the dry mass consumed by larvae. The chemical profile of flavonoids from each genotype was assessed by means of LC-MS. Based on the results of the weevil biological parameters and dry mass consumed provided by univariate and multivariate statistical analyses, the bean genotypes were classified into four levels of resistance (antibiosis): highly resistant, moderately resistant, susceptible and highly susceptible. Among the evaluated flavonoids, we could identify isoquercitrin; however, it has no correlation with the expression of resistance. From the results obtained in this work, more studies will be conducted with the genotypes that stood out as resistant, evaluating other resistance categories and defense mechanisms of these materials against the attack of Z. subfasciatus and other pests, as well as studies of other important agronomic characteristics aiming at future commercialization of the bean genotypes. Graphical abstract: Principal component analysis (PCA) for common bean genotypes and time (days) for incubation, egg to adult emergence (Egg to adult), larvae hatching to adult emergence (Larva to adult), longevity, egg to adult death (life cycle) and egg viability (%), emerged adults (%), adult weight (mg), sex ratio and consumed dry mass (mg), for Zabrotes subfasciatus . Highlights: Forty-three common bean genotypes were assessed for resistance to Zabrotes subfasciatus. The genotypes were sorted into four resistance levels. 283, 361, 179, 282, 284, 186, 173, 364, Raz 59, 177 and Raz 49 are resistant genotypes. No statistical correlation between isoquercitrin and genotype resistance was found.