Paxillus rubicundulus (Boletales, Paxillaceae) and two new alder-specific ectomycorrhizal species, Paxillus olivellus and Paxillus adelphus, from Europe and North Africa. Issue 5 (May 2016)

• Record Type: Journal Article
• Authors: Jargeat, Patricia; Moreau, Pierre-Arthur; Gryta, Hervé; Chaumeton, Jean-Paul; Gardes, Monique
• Is Part Of: Fungal biology. Volume 120:Issue 5(2016:May)
• Page Start: 711
• Page End: 728
• Publication Date: 2016-05
• Subjects: Alnus -- Ectomycorrhizal fungi -- Molecular phylogeny -- Taxonomy; Mycology -- Periodicals; Fungi -- Periodicals; 579.505
• DOI: 10.1016/j.funbio.2016.02.008
• Languages: English
• ISSNs: 1878-6146

Abstract

Abstract: Paxillus rubicundulus P.D. Orton has been shown to be a complex of at least three ectomycorrhizal taxa strictly associated with alders ( Alnus ) in Europe, P. rubicundulus s. str., and two undescribed clades. To assess the taxonomic status of these three clades and their phylogenetic relationships, phylogenetic analyses of two independent gene regions (ITS and gpd ), combined with macro- and micromorphological comparisons of genetically identified specimens, were carried out. A total of 85 sequences were successfully obtained from basidiomata and alder mycorrhizae collected in France and Algeria and combined with GenBank and UNITE sequences. The phylogenetic results and estimates of genetic diversity confirmed that the three clades are distinct species, often found in sympatry. As a result, P. rubicundulus s. str. was redefined based on the revision of type material, and Paxillus adelphus and Paxillus olivellus are introduced as new Linnaean names. The often used name Paxillus filamentosus is rejected since it could not be applied to any of the new species. The three species are distinguished micromorphologically by spore size and shape. They are widely distributed in Europe, North Africa and western Asia; P. rubicundulus is rare, and all species have a limited host range. Highlights: Species boundaries in the Paxillus rubicundulus complex were investigated. Two phylospecies were taxonomically described and named Paxillus adelphus sp. nov. and Paxillus olivellus sp. nov. The most common name Paxillus filamentosus could not be applied to any of the two new species. Paxillus rubicundulus s. str. was redefined based on the revision of type material. The three alnicolous species are widely distributed in the temperate Old World but seem to be absent in the New World.