Sustained presentation of BMP‐2 enhances osteogenic differentiation of human adipose‐derived stem cells in gelatin hydrogels. Issue 6 (18th February 2016)

• Record Type: Journal Article
• Authors: Samorezov, Julia E.; Headley, Emma B.; Everett, Christopher R.; Alsberg, Eben
• Is Part Of: Journal of biomedical materials research. Volume 104:Issue 6(2016)
• Page Start: 1387
• Page End: 1397
• Publication Date: 2016-02-18
• Subjects: bone tissue engineering -- drug delivery -- growth factor -- gelatin methacrylate -- biomaterials; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35668
• Languages: English
• ISSNs: 1549-3296

Abstract

Abstract: Human adipose‐derived stem cells (hASCs) show great potential for healing bone defects. Bone morphogenetic protein‐2 (BMP‐2) has been reported to stimulate their osteogenic differentiation both in vitro and in vivo . Here, methacrylated gelatin (GelMA) hydrogels were evaluated as a system to deliver BMP‐2 to encapsulated hASCs from two different donors, and BMP‐2 delivered from the hydrogels was compared to BMP‐2 presented exogenously in culture media. GelMA hydrogels were shown to provide sustained, localized presentation of BMP‐2 due to electrostatic interactions between the growth factor and biomaterial after an initial burst release. Both donors exhibited similar responses to the loaded and exogenous growth factor; BMP‐2 from the hydrogels had a statistically significant effect on hASC osteogenic differentiation compared to exogenous BMP‐2. Expression of alkaline phosphatase was accelerated, and cells in hydrogels with loaded BMP‐2 deposited more calcium at one, two, and four weeks than cells without BMP‐2 or with the growth factor presented in the media. There were no statistically significant differences in calcium content between groups with 25, 50, or 100 µg/mL loaded BMP‐2, suggesting that using a lower growth factor dose may be as effective as a higher loading amount in this system. Taken together, these findings suggest that controlled delivery of BMP‐2 from the GelMA enhances its osteogenic bioactivity compared to free growth factor presented in the media. Thus, the GelMA system is a promising biomaterial for BMP‐2‐mediated hASC osteogenesis. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1387–1397, 2016.