A Laboratory Exercise for Genotyping Two Human Single Nucleotide Polymorphisms. Issue 1 (2nd January 2016)

• Record Type: Journal Article
• Authors: Fernando, James; Carlson, Bradley; LeBard, Timothy; McCarthy, Michael; Umali, Finianne; Ashton, Bryce; Rose, Ferrill F.
• Is Part Of: Journal of biological education. Volume 50:Issue 1(2016)
• Page Start: 73
• Page End: 85
• Publication Date: 2016-01-02
• Subjects: Single nucleotide polymorphism -- DNA sequencing -- Bitter taste -- Genomics -- Genetic variation; Biology -- Study and teaching -- Periodicals; Biologie -- Étude et enseignement -- Périodiques; Biology -- Study and teaching; Periodicals; 570.7
• DOI: 10.1080/00219266.2015.1007885
• Languages: English
• ISSNs: 0021-9266

Abstract

Abstract : The dramatic decrease in the cost of sequencing a human genome is leading to an era in which a wide range of students will benefit from having an understanding of human genetic variation. Since over 90% of sequence variation between humans is in the form of single nucleotide polymorphisms (SNPs), a laboratory exercise has been devised in order to illustrate the importance of SNPs. Two separate SNPs are analysed, one of which has a significant effect on a person's phenotype and one which does not. The genotyping protocol is relatively inexpensive and uses standard molecular biology reagents and equipment.