DNA interaction, antitumor and antimicrobial activities of three-dimensional chitosan ring produced from the body segments of a diplopod. (1st August 2016)

• Record Type: Journal Article
• Authors: Kaya, Murat; Akyuz, Bahar; Bulut, Esra; Sargin, Idris; Tan, Gamze; Erdonmez, Demet; Maheta, Mansi; Satkauskas, Saulius; Mickevičius, Saulius
• Is Part Of: Carbohydrate polymers. Volume 146(2016)
• Page Start: 80
• Page End: 89
• Publication Date: 2016-08-01
• Subjects: Millipede -- Chitin -- Biopolymer -- Cytotoxicity -- HeLa cell line -- Antibacterial; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.03.033
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Ring shaped and flexible chitin and chitosan was obtained from diplopod body segments. This new three-dimensional chitosan consists of nanofibers and nanopores. Characterization of three-dimensional chitin and chitosan. Interaction of 3D chitosan with Plasmid DNA. Antitumoral and antimicrobial activities of the chitosan were investigated. Abstract: Commercially available chitins and the chitin isolated from mushrooms, insect cuticles, shells of shrimp, crab and crayfish reported in the literature are in forms of powder, flake or granule. Three-dimensional chitins have been only known from the sponges but still three-dimensional chitosan has not been reported yet. In this study, we produced three-dimensional chitin and chitosan rings from the body segments of a diplopod species ( Julus terrestris ). Obtained chitin and chitosan rings were characterized (by FT-IR, SEM, TGA, XRD, dilute solution viscometry and EA) and compared with commercial chitin and chitosan. The interactions with plasmid DNA was studied at varying concentrations of chitosan (0.04, 0.4 and 4 mg/mL). Antitumor activity tests were conducted (L929 and HeLa), low cytotoxicity and high antiproliferative activity was observed. Antimicrobial activities of J . terrestris chitosan were investigated on twelve microorganisms and maximum inhibition (15.6 ± 1.154 mm) was recorded for common human pathogen Staphylococcus aureus .