Re‐evaluation of HNA‐1 antibodies for their reactivity to different recombinant variants of FcγRIIIb. (28th December 2015)

• Record Type: Journal Article
• Authors: Nishizawa, K.; Yasui, K.; Amakishi, E.; Irie, Y.; Matsuyama, N.; Kamada, H.; Nakajima, F.; Ishii, H.; Matsukura, H.; Hirayama, F.
• Is Part Of: ISBT science series. Volume 11:Number 1(2016)
• Page Start: 45
• Page End: 50
• Publication Date: 2015-12-28
• Subjects: antibody -- HNA‐1 -- polymorphism; Blood -- Periodicals; Blood -- Transfusion -- Periodicals; Immunohematology -- Periodicals; Immunopathology -- Periodicals; 615.39
• DOI: 10.1111/voxs.12239
• Languages: English
• ISSNs: 1751-2816

Abstract

Abstract : Background and Objectives: The Fcgamma receptor IIIb (Fc γ RIIIb) carries the human neutrophil antigen (HNA)‐1 system. The three FCGR3B alleles encode four antigens: one allele encodes HNA‐1a, and two alleles encode two antigens each, HNA‐1b and HNA‐1d and HNA‐1b and HNA‐1c, respectively. Several other non‐synonymous substitutions have also been reported. Little is known about the properties of HNA‐1d antibodies because HNA‐1d was recently discovered, and HNA‐1d antibodies have been reported only in a couple of cases. These findings raise the possibility that the polymorphisms in the HNA‐1 system and spectrum of the specificity of resulting antibodies are more complex than previously thought. We address this possibility in this work. Materials and Methods: Several HNA‐1 antibodies, which had been classified as HNA‐1a or HNA‐1b before HNA‐1d was discovered, were re‐evaluated for their reactivity to different recombinant variants of Fc γ RIIIb. Results: The HNA‐1a antibody, but not the reference HNA‐1a antibody, cross‐reacted to HNA‐1d. Further, nucleotide replacement in FCGR3B alleles *02 at 193G→A (AA position 54 E→K) resulted in the disappearance of HNA‐1d, but not HNA‐1b, antigenicity. Conclusion: The HNA‐1 system may be more complex than previously understood.