Synthesis of 24‐h soluble gelatin sponge particles and their effect on liver necrosis following hepatic artery embolization: Results of in vitro and in vivo studies. Issue 4 (2nd July 2015)

• Record Type: Journal Article
• Authors: Sanda, Hiroki; Kawai, Nobuyuki; Sato, Morio; Tanaka, Fumihiro; Ikoma, Akira; Nakai, Motoki; Minamiguchi, Hiroki; Sonomura, Tetsuo; Matsuzaki, Ibu; Warigaya, Kenji; Murata, Shinichi
• Is Part Of: Hepatology research. Volume 46:Issue 4(2016)
• Page Start: 335
• Page End: 342
• Publication Date: 2015-07-02
• Subjects: hepatic artery embolization -- hepatocellular carcinoma -- soluble gelatin sponge particles; Liver -- Diseases -- Periodicals; Liver Diseases -- Periodicals; Foie -- Maladies -- Périodiques; 616.362
• DOI: 10.1111/hepr.12539
• Languages: English
• ISSNs: 1386-6346

Abstract

Abstract : Aim: To synthesize 24‐h soluble gelatin sponge particles (SGSP) of 200–500 and 500–1000 µm, and to investigate their ischemic potency following hepatic artery embolization (HAE). Methods: Low‐endotoxin gelatin was freeze‐dried and heated at 110, 115, 118, 120, 122 and 125°C to form cross‐linked gelatin sponge. We prepared 200–500‐ and 500–1000‐µm SGSP by pulverizing and sieving the gelatin sponge. The dissolution times in saline were measured. Eight healthy pigs underwent HAE of the right and left hepatic arteries with either 200–500‐ or 500–1000‐µm SGSP ( n = 4/group). Results: The particles prepared at 110–122°C were soluble whereas particles prepared at 125°C or more were insoluble. The mean dissolution time of the particles increased with increasing temperature. In each pig, sequential arteriography confirmed that recanalization was complete 24 h after embolization. Pathological tests 48 h after HAE revealed coagulation necrosis but least damage to the biliary tract. The liver necrosis rate (mean ± standard deviation) was significantly greater in the 200–500‐µm group than in the 500–1000‐µm group (9.89 ± 4.04% vs 4.44 ± 0.67%, respectively; P = 0.0027). A significantly greater proportion of arteries with a diameter of 100–200 µm had residual SGSP in the 200–500‐µm group than in the 500–1000‐µm group ( P < 0.002). Conclusion: HAE with 200–500‐µm SGSP had greater effects on promoting liver necrosis without biliary damage than did HAE with 500–1000‐µm SGSP.