Structural characterization of NRAS isoform 5. (24th March 2016)

• Record Type: Journal Article
• Authors: Markowitz, Joseph; Mal, Tapas K.; Yuan, Chunhua; Courtney, Nicholas B.; Patel, Mitra; Stiff, Andrew R.; Blachly, James; Walker, Christopher; Eisfeld, Ann‐Kathrin; de la Chapelle, Albert; Carson, William E.
• Is Part Of: Protein science. Volume 25:Number 5(2016:May)
• Page Start: 1069
• Page End: 1074
• Publication Date: 2016-03-24
• Subjects: melanoma -- NMR -- isoform -- NRAS; Proteins -- Periodicals; 572.6
• DOI: 10.1002/pro.2916
• Languages: English
• ISSNs: 0961-8368

Abstract

Abstract: It was recently discovered that the NRAS isoform 5 (20 amino acids) is expressed in melanoma and results in a more aggressive cell phenotype. This novel isoform is responsible for increased phosphorylation of downstream targets such as AKT, MEK, and ERK as well as increased cellular proliferation. This structure report describes the NMR solution structure of NRAS isoform 5 to be used as a starting point to understand its biophysical interactions. The isoform is highly flexible in aqueous solution, but forms a helix‐turn‐coil structure in the presence of trifluoroethanol as determined by NMR and CD spectroscopy. Abstract : PDB Code(s):2n9c