Recovery of terrestrial plants in vegetative vigor and seedling emergence tests from exposure to atrazine. (16th February 2016)

• Record Type: Journal Article
• Authors: Brain, Richard A.; Hoberg, James
• Is Part Of: Environmental toxicology and chemistry. Volume 35:Number 5(2016:May)
• Page Start: 1284
• Page End: 1296
• Publication Date: 2016-02-16
• Subjects: Herbicide -- Plant -- Risk assessment -- Phytotoxicity -- Recovery -- Atrazine; Pollution -- Environmental aspects -- Periodicals; Environmental chemistry -- Periodicals; 615.902
• DOI: 10.1002/etc.3298
• Languages: English
• ISSNs: 0730-7268

Abstract

Abstract: Ten species of terrestrial plants, including 6 dicotyledonous and 4 monocotyledonous species, were exposed to a direct overspray of atrazine according to US Environmental Protection Agency seedling emergence and vegetative vigor study guidelines and subsequently evaluated for potential recovery. For each species, no‐observed‐effect rate (NOER), 10% effect rate, 25% effect rate, and 50% effect rate values were calculated (where possible) for a variety of guideline‐required endpoints (but focusing on growth rate) for both the standard experimental phase and a recovery phase; and the rates subsequently were compared. For the seedling emergence study, the standard experimental (designated test 1) and recovery (designated test 2) phases encompassed days 0 to 14 and days 14 to 28, respectively. Similarly, for the vegetative vigor study, test 1 and test 2 encompassed days 0 to 21 and days 21 to 42, respectively. Plants were exposed to atrazine at nominal application rates ranging from 1.1 g active ingredient (a.i.)/ha (0.0010 lb a.i./A) to 28 000 g a.i./ha (25 lb a.i./A), depending on the species; the 28 000 g a.i./ha rate is greater than 12 times the maximum application rate of 2250 g a.i./ha (2 lb a.i./A) registered on corn. For seedling emergence, only 2 of 10 species tested, cabbage and tomato, provided clear rate responses in the initial 14 d of exposure (test 1). Based on a comparison of x % effect rate (ER x ) and NOER values for growth rates of shoot length and shoot dry weight for days 0 to 14 relative to days 14 to 28, recovery was apparent for cabbage shoot length growth rate and tomato shoot length and shoot dry weight growth rates. Test application rates selected for the remaining 8 species showed either a weak response that did not allow a clear assessment of recovery or no response at all. For the vegetative vigor study, 9 of the 10 species tested provided clear rate responses in test 1 (days 0–21); corn did not demonstrate any herbicidal response up to the highest rate tested, 28 000 g a.i./ha. Based on comparison of day 0 to 21 (test 1) relative to day 21 to 42 (test 2) ER x and NOER values for shoot length, average growth rates indicated that 8 of 9 species clearly demonstrated an increase in 2 or more metrics (cabbage did not demonstrate a response based on shoot length). Clear recovery was also indicated by an increase in ER x and/or NOER values from test 1 to test 2 for shoot dry weight average growth rates for 7 of the 9 species (corn did not show a response, and oat and soybean showed variable responses). Thus, in most species, where initial herbicidal effects were observed, the effects are largely ameliorated over time. Environ Toxicol Chem 2016;35:1284–1296. © 2015 SETAC