A high-throughput DNA extraction method from rice seeds. Issue 1 (2nd January 2016)

• Record Type: Journal Article
• Authors: Liang, Huabing; Deng, Ya; Wang, Chuntai; Xu, Xin
• Is Part Of: Biotechnology, biotechnological equipment. Volume 30:Issue 1(2016)
• Page Start: 32
• Page End: 35
• Publication Date: 2016-01-02
• Subjects: DNA extraction -- rice -- seeds; Biotechnology -- Periodicals; Biotechnology -- Periodicals; Biotechnology -- instrumentation -- Periodicals; Periodicals; 660.605
• DOI: 10.1080/13102818.2015.1088401
• Languages: English
• ISSNs: 1310-2818

Abstract

ABSTRACT: Rapid and inexpensive preparation of genomic DNA from rice seeds for marker-assisted selection and seed purity estimation is a major bottleneck for plant breeders. Here, we describe a high-throughput method that provides DNA at sufficient quantity and quality for these applications. Optimization of buffer composition and individual protocol stages allow processing of 384 samples within 2 h, yielding templates that reliably support downstream polymerase chain reaction of single copy amplicons up to 1.2 kb.