Urinary neutrophil gelatinase‐associated lipocalin concentration changes after acute haemorrhage and colloid‐mediated reperfusion in anaesthetized dogs. (15th October 2015)

• Record Type: Journal Article
• Authors: Davis, Jennifer; Raisis, Anthea L; Cianciolo, Rachel E; Miller, David W; Shiel, Robert E; Nabity, Mary B; Hosgood, Giselle L
• Is Part Of: Veterinary anaesthesia and analgesia. Volume 43:Number 3(2016:May)
• Page Start: 262
• Page End: 270
• Publication Date: 2015-10-15
• Subjects: acute kidney injury -- dogs -- hypotension -- ischaemia -- NGAL; Veterinary anesthesia -- Periodicals; 636.089
• DOI: 10.1111/vaa.12311
• Languages: English
• ISSNs: 1467-2987

Abstract

Abstract: Objective: To determine changes in urine neutrophil gelatinase‐associated lipocalin concentration (uNGAL) in anaesthetized Greyhound dogs that developed acute tubular damage following haemorrhage and resuscitation with colloid‐based fluids. Study design: Prospective experimental study. Animals: Seven healthy adult entire male Greyhound dogs. Methods: During isoflurane anaesthesia, approximately 50 mL kg −1 of blood was removed to maintain mean arterial pressure (MAP) ≤40 mmHg for 1 hour followed by gelatin‐based colloid administration to maintain MAP ≥60 mmHg for 3 hours. Data, including oxygen extraction ratio and uNGAL, were collected before (T0) and immediately following (T1) haemorrhage, and hourly during reperfusion (T2–T4). After T4, dogs were euthanized and renal tissue was collected for histology. Statistical analysis was performed using repeated‐measures one‐wayanova . Data are presented as means (95% confidence interval). Results: Histology identified renal tubular epithelial damage in all dogs. Urine NGAL concentration increased from 12.1 (0–30.6) ng mL −1 at T0 to 122.0 (64.1–180.0) ng mL −1 by T3. Compared with T0, uNGAL was significantly higher at T3 ( p = 0.016) and was increased 24‐fold. Conclusions and clinical relevance: Despite wide individual variation in baseline uNGAL, increases in uNGAL were observed in all dogs, suggesting that this biomarker has the potential to detect renal tubular injury following haemorrhage‐induced hypotension and colloid‐mediated reperfusion.