Post-translational control of protein function with light using a LOV-intein fusion protein. Issue 4 (4th March 2016)

• Record Type: Journal Article
• Authors: Jones, D. C.; Mistry, I. N.; Tavassoli, A.
• Is Part Of: Molecular bioSystems. Volume 12:Issue 4(2016:Apr.)
• Page Start: 1388
• Page End: 1393
• Publication Date: 2016-03-04
• Subjects: Molecular biology -- Periodicals; Biochemistry -- Periodicals; 571.7405
• DOI: 10.1039/c6mb00007j
• Languages: English
• ISSNs: 1742-206X

Abstract

Abstract : Modified split-inteins are fused to the photosensitive LOV2 domain to enable photoactivated cis intein splicing. Abstract : Methods for the post-translational control of protein function with light hold much value as tools in cell biology. To this end, we report a fusion protein that consists of DnaE split-inteins, flanking the light sensitive LOV2 domain of Avena sativa . The resulting chimera combines the activities of these two unrelated proteins to enable controlled formation of a functional protein via upregulation of intein splicing with blue light in bacterial and human cells.