Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae. Issue 6 (4th November 2015)

• Record Type: Journal Article
• Authors: Zhang, Lijie; Liu, Qiuyuan; Ge, Yongsheng; Li, Lixiang; Gao, Chao; Xu, Ping; Ma, Cuiqing
• Is Part Of: Green chemistry. Volume 18:Issue 6(2016)
• Page Start: 1560
• Page End: 1570
• Publication Date: 2015-11-04
• Subjects: Environmental chemistry -- Industrial applications -- Periodicals; Environmental management -- Periodicals; 660
• DOI: 10.1039/c5gc01638j
• Languages: English
• ISSNs: 1463-9262

Abstract

Abstract : Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae . Abstract : Acetoin (AC) is regarded as one of the top potential sugar-derived chemical building blocks that can be used as food additives, precursors in chemical synthesis, and plant growth promoting molecules. In this study, a low-cost lignocellulosic resource of pretreated corn stover was used as a carbon source to produce AC. After redirecting the metabolic flux, fine tuning reducing power, and eliminating carbon catabolite repression in Enterobacter cloacae SDM, a systematically engineered strain SDM 53 was constructed, which is able to utilize glucose and xylose efficiently and simultaneously. Using fed-batch fermentation of SDM 53, 45.6 g L −1 AC was produced at a rate of 1.52 g L −1 h −1 using the lignocellulosic hydrolysate. Biotechnological synthesis of AC has various advantages such as being sustainable and environment-friendly. With its desirable properties, the engineered strain SDM 53 may be a potential choice for the industrial production of AC.