Direct Electrochemical Sensing of Phenazine‐1‐carboxylic Acid Secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057T Using Disposable Screen‐printed Carbon Electrode. Issue 4 (24th November 2015)

• Record Type: Journal Article
• Authors: Hameed, Asif; Pi, Hong‐Wei; Lin, Shih‐Yao; Lai, Wei‐An; Young, Li‐Sen; Liu, You‐Cheng; Shen, Fo‐Ting; Young, Chiu‐Chung
• Is Part Of: Electroanalysis. Volume 28:Issue 4(2016)
• Page Start: 846
• Page End: 853
• Publication Date: 2015-11-24
• Subjects: Phenazine -- Cyclic voltammetry -- Electropolymerization -- Preanodization -- Pseudomonas aeruginosa; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201500278
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: A novel electrochemical approach for direct recognition of antibiotic phenazine‐1‐carboxylic acid (PCA) was developed. PCA was electropolymerized on preanodized screen‐printed carbon electrode (SPCE*‐PCA) through repetitive cyclic voltammetry and characterized by XPS and FESEM. Electron transfer involved intermediate phenomenon of diffusion‐controlled redox process and surface bound redox reaction. At pH 8 (optimum), SPCE*‐PCA had a detection limit of 0.51±0.04 μM, a quantification limit of 1.7±0.13 μM, linearity of up to 50 µM, a repeatability of 15.5 % and a reproducibility of 1.7 %. PCA secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057 T was investigated successfully using present single run approach. Abstract :