Quantification of leucocytes, T-lymphocytes and macrophages in autoptical endomyocardial tissue from 56 normal human hearts during the first year of life. (May 2016)

• Record Type: Journal Article
• Authors: Grasmeyer, Sarah; Oswald, Sylvia; Madea, Burkhard
• Is Part Of: Forensic science international. Volume 262(2016)
• Page Start: 108
• Page End: 112
• Publication Date: 2016-05
• Subjects: Endomyocardial autoptical samples -- Myocarditis -- Normal hearts -- Infants -- First year of life; Medical jurisprudence -- Periodicals; Chemistry, Forensic -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Chimie légale -- Périodiques; Gerechtelijke geneeskunde; Gerechtelijke chemie; Gerechtelijke psychiatrie; Chemistry, Forensic; Medical jurisprudence; Electronic journals; Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.forsciint.2016.02.049
• Languages: English
• ISSNs: 0379-0738

Abstract

Graphical abstract: Highlights: Myocardial inflammatory cell counts in the first year of life were assessed. Two counting methods were compared. Cell counts differed considerably between individual HPFs and individual samples. Cell counts were independent from child age and heart weight. Abstract: This study evaluated the normal number of inflammatory cells in the heart in the first year of life using two methods to compare their ability to quantitate physiological myocardial infiltration. Eight endomyocardial samples from both ventricles were obtained at autopsy from 56 structurally normal hearts during the first year of life. In each sample the numbers of leucocytes, T-lymphocytes and macrophages were counted once in 20 randomly chosen high-power fields (400×) as well as in a 10 mm 2 area of randomly chosen myocardial tissue (100×) by two independent investigators. Compared to the literature a greater representative proportion of myocardial tissue was analyzed. The results of the enumeration in mm 2 were converted into high-power-fields to compare both methods. The mean numbers and standard deviations for leucocytes, T-lymphocytes and macrophages were calculated. Both counting methods showed similar results with low inflammatory cell counts per single heart and staining. A greater understanding of the physiological myocardial infiltration by leucocytes, T-lymphocytes and macrophages is important for postmortem forensic cases, and for the interpretation of endomyocardial biopsies in infants.