Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions. Issue 3 (11th December 2015)

• Record Type: Journal Article
• Authors: Tang, Shan; Wan, Zhengpeng; Gao, Yiren; Zheng, Ji-Shen; Wang, Jing; Si, Yan-Yan; Chen, Xin; Qi, Hai; Liu, Lei; Liu, Wanli
• Is Part Of: Chemical science. Volume 7:Issue 3(2016:Mar.)
• Page Start: 1891
• Page End: 1895
• Publication Date: 2015-12-11
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5sc03404c
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen–antibody interaction mediated responses in B cells. Abstract : We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen–antibody interaction mediated responses in B cells. This strategy facilitated fine tuning of the caged protein antigen to optimize its bioactivity and photochemical properties. One optimal molecule, HEL-K96 NPE, was totally inert to hen egg lysozyme (HEL)-specific B cells and could only restore its antigenicity upon photoactivation. Combined with real time live cell imaging, the utility of HEL-K96 NPE was demonstrated as a proof of concept to quantify B cell synapse formation and calcium influx responses at the single cell level.