Detection of total protein in milk using phosphomolybdic acid‐mediated surface‐enhanced Raman spectroscopy. (12th October 2015)

• Record Type: Journal Article
• Authors: Huang, Yingzhao; Gu, Jianming; Xiang, Gang; Xu, Jiajie; Fu, Shuilin; Gong, Heng
• Is Part Of: Journal of Raman spectroscopy. Volume 47:Number 3(2016)
• Page Start: 277
• Page End: 282
• Publication Date: 2015-10-12
• Subjects: total protein -- casein -- milk -- phosphomolybdic acid -- SERS; Raman spectroscopy -- Periodicals; 535.846
• DOI: 10.1002/jrs.4812
• Languages: English
• ISSNs: 0377-0486

Abstract

Abstract : Although many surface‐enhanced Raman scattering (SERS)‐based methods for detecting specific proteins have been studied, simple and direct detection of total protein in liquid using a SERS‐based method remains difficult. In this study, a distinguishable effect on the SERS spectra from pre‐mixture of phosphomolybdic acid (PMA) with protein was found, indicating that PMA could be used as a SERS reporter for total protein detection in a liquid sample. Further experiments confirmed a good linear relationship between a premixed concentration of protein (casein, whey protein or bovine serum albumin) and the SERS intensity of PMA in our SERS system. Using casein as a reference, a PMA‐mediated SERS method was proposed that can quantitatively analyze protein at 2.5–25 µg/ml with a limit of detection of 1.5 µg/ml. Our PMA‐mediated SERS method is a simple and rapid method for quantitative analysis of total protein in milk. Copyright © 2015 John Wiley & Sons, Ltd. Abstract : A distinguishable effect on the SERS spectra from pre‐mixture of phosphomolybdic acid (PMA) with protein was found, indicating that PMA could be used as a SERS reporter for protein detection in a liquid sample. Using casein as a reference, a PMA‐mediated SERS method was proposed that can quantitatively analyze protein at 2.5–25 µg/ml with a limit of detection of 1.5 µg/ml. Our PMA‐mediated SERS method is a simple and rapid method for quantitative analysis of total protein in milk.