Structural and Genetic Diversity of Hexavalent Chromium-Resistant Bacteria in Contaminated Soil. Issue 3 (15th March 2016)

• Record Type: Journal Article
• Authors: Wang, Yangyang; Chai, Liyuan; Liao, Qi; Tang, Chongjian; Liao, Yingping; Peng, Bing; Yang, Zhihui
• Is Part Of: Geomicrobiology journal. Volume 33:Issue 3/4(2016)
• Page Start: 222
• Page End: 229
• Publication Date: 2016-03-15
• Subjects: Bacterial biodiversity -- DGGE -- genetic diversity -- hexavalent chromium -- rep-PCR -- soil contamination; Geomicrobiology -- Periodicals; Biogeochemistry -- Periodicals; 579
• DOI: 10.1080/01490451.2015.1054006
• Languages: English
• ISSNs: 0149-0451

Abstract

ABSTRACT: Culture-independent and -dependent methods were used to evaluate the genetic diversity of hexavalent chromium-resistant bacteria in contaminated soils. Denaturing gradient gel electrophoresis (DGGE) showed that hexavalent chromium-resistant bacteria in contaminated soils had significant structural diversity that was not related to the concentration of hexavalent chromium, while the diversity of soil bacterial communities was correlated with soil pH. Twenty-two chromium-resistant bacterial strains were isolated from contaminated soil, including members of Brevundimonas, Micrococcus, Exiguobacterium, Alcaligenes and Pannonibacter . Repetitive sequence-based polymerase chain reaction showed that the BOX- primer produced the more complex banding patterns than the ERIC- and REP- primers, indicating a high degree of genotypic diversity. These bacterial strains varied in ability to reduce hexavalent chromium. Pannonibacter reduced 300 mg·L −1 of hexavalent chromium in solution within 24 h. X-ray photoelectron spectra (XPS) analysis revealed that Cr(OH)3, Cr2 O3, and CrCl3 composed approximately 83.51% of the total chromium in the reduction product.