Comparison between the additive effects of diluted (rFSH) and diluted/dynamized (FSH 6 cH) recombinant follicle-stimulating hormone on the in vitro culture of ovine preantral follicles enclosed in ovarian tissue. (April 2016)

• Record Type: Journal Article
• Authors: Lima, L.F.; Rocha, R.M.P.; Alves, A.M.C.V.; Carvalho, A.A.; Chaves, R.N.; Lopes, C.A.P.; Báo, S.N.; Campello, C.C.; Rodrigues, A.P.R.; Figueiredo, J.R.
• Is Part Of: Complementary therapies in medicine. Volume 25(2016)
• Page Start: 39
• Page End: 44
• Publication Date: 2016-04
• Subjects: Homeopathy -- Ovine -- Ovary -- Reproduction; Alternative medicine -- Periodicals; Complementary Therapies -- Periodicals; Médecines parallèles -- Périodiques; Thérapeutique -- Périodiques; Alternative medicine; Electronic journals; Periodicals; 615.5
• DOI: 10.1016/j.ctim.2015.12.016
• Languages: English
• ISSNs: 0965-2299

Abstract

Highlights: We compared 2 types of recombinant follicle stimulating hormone. Fifty nanogram per liter of diluted rFSH induced higher rates of follicular survival and growth. Diluted/dynamized FSH induced higher follicular diameter and survival rate. Diluted/dynamized FSH may be used as an alternative to diluted rFSH. Abstract: Objective: This study compared 2 types of recombinant follicle stimulating hormone (rFSH): diluted and diluted/dynamized, on in vitro development of ovine follicles. Methods: In experiment 1, ovarian fragments were cultured for 1 or 7 days in α-MEM + in the absence or presence of different concentrations of diluted rFSH to determine the best concentration. In experiment 2, the effect of diluted and diluted/dynamized rFSH (rFSH 6 cH—ultradiluted and succussioned), alone or in combination, was studied. Results: In experiment 1, compared to control, 50 ng/mL of diluted rFSH induced higher rates of follicular survival after 7 days of culture and higher percentages of growing follicles at day 1 of culture ( P < 0.05). In experiment 2, compared to control, diluted/dynamized rFSH induced higher follicular diameter and survival rate after 7 days and early follicle activation at day 1 of culture ( P < 0.05). Compared to diluted rFSH, diluted/dynamized rFSH induced higher rates of follicle activation at day 1 of culture ( P < 0.05). Conclusion: In conclusion, compared to the control medium, diluted/dynamized rFSH promoted survival and early activation of follicles, while diluted rFSH promoted higher activation later in the culture. Thus, diluted/dynamized rFSH may be used as an alternative to diluted rFSH for the in vitro culture of ovine preantral follicles.