Superparamagnetic iron oxide nanoparticles for direct labeling of stem cells and in vivo MRI tracking. (3rd August 2015)

• Record Type: Journal Article
• Authors: Kim, Saejeong J.; Lewis, Bobbi; Steiner, Mark‐ Steven; Bissa, Ursula V.; Dose, Christian; Frank, Joseph A.
• Is Part Of: Contrast media & molecular imaging. Volume 11:Number 1(2016:Jan./Feb.)
• Page Start: 55
• Page End: 64
• Publication Date: 2015-08-03
• Subjects: superparamagnetic iron oxide nanoparticles -- dextran -- transfection reagent free or direct labeling -- bone marrow stromal cells -- neural stem cells -- hematopoietic stem; Diagnostic imaging -- Periodicals; Magnetic resonance imaging -- Periodicals; Contrast media (Diagnostic imaging) -- Periodicals; Contrast Media -- Periodicals; Diagnostic Imaging -- Periodicals; Substances de contraste -- Périodiques; Diagnostics moléculaires -- Périodiques; Imagerie médicale; Substance de contraste; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 616.0754
• DOI: 10.1002/cmmi.1658
• Languages: English
• ISSNs: 1555-4309

Abstract

Abstract : To develop effective stem cell therapies, it is important to track therapeutic cells non‐invasively and monitor homing to areas of pathology. The purpose of this study was to1 design and evaluate the labeling efficiency of commercially available dextran‐coated superparamagnetic iron oxide nanoparticles, FeraTrack Direct (FTD), in various stem and immune cells;2 assess the cytotoxicity and tolerability of the FTD in stem cells; and3 monitor stem cell homing using FTD‐labeled bone‐marrow‐derived mesenchymal stromal cells (BMSCs) and neural stem cells (NSCs) in a tumor model by in vivo MRI. BMSCs, NSCs, hematopoietic stem cells (HSCs), T‐lymphocytes, and monocytes were labeled effectively with FTD without the need for transfection agents, and Prussian blue (PB) staining and transmission electron microscopy (TEM) confirmed intracellular uptake of the agent. The viability, proliferation, and functionality of the labeled cells were minimally or not affected after labeling. When 10 6 FTD‐labeled BMSCs or NSCs were injected into C6 glioma bearing nude mice, the cells homing to the tumors were detected as hypointense regions within the tumor using 3 T clinical MRI up to 10 days post injection. Histological analysis confirmed the homing of injected cells to the tumor by the presence of PB positive cells that are not macrophages. Labeling of stem cells or immune cells with FTD was non‐toxic, and should facilitate the translation of this agent to clinical trials for evaluation of trafficking of cells by MRI. Copyright © 2015 John Wiley & Sons, Ltd. Abstract : Labeling of various stem and immune cells with FeraTrack Direct was efficient and minimally toxic. BMSCs and NSCs labeled with FeraTrack Direct were tracked for cell homing in a mouse flank tumor model with 3 T MRI.