Fasted/fed states regulate postsynaptic hub protein DYNLL2 and glutamatergic transmission in oxytocin neurons in the hypothalamic paraventricular nucleus. (April 2016)

• Record Type: Journal Article
• Authors: Suyama, Shigetomo; Kodaira-Hirano, Misato; Otgon-Uul, Zesemdorj; Ueta, Yoichi; Nakata, Masanori; Yada, Toshihiko
• Is Part Of: Neuropeptides. Volume 56(2016)
• Page Start: 115
• Page End: 123
• Publication Date: 2016-04
• Subjects: Oxytocin -- Paraventricular nucleus -- Fasting -- Feeding -- Synaptic plasticity -- NMDA receptor -- Slice patch clamp -- DYNLL2 -- Proteomics; Neuropeptides -- Periodicals; Neuropeptides; Neuropeptides -- Périodiques; Neuropeptides; Electronic journals; Periodicals; 572.65
• DOI: 10.1016/j.npep.2015.08.008
• Languages: English
• ISSNs: 0143-4179

Abstract

Abstract: The neurons in the hypothalamus regulate food intake and energy metabolism on reception of systemic energy states. Accumulating evidences have indicated that synaptic transmission on the hypothalamic neurons is modulated by the metabolic condition related to fasted/fed states, and that this modulation of synaptic plasticity plays a role in regulation of feeding. It has been shown that oxytocin (Oxt) neurons in the paraventricular nucleus (PVN) of the hypothalamus sense and integrate various peripheral and central signals and thereby induce satiety. However, whether metabolic conditions regulate the synaptic transmission on Oxt neurons in PVN remains unclear. The present study examined whether the fasted/fed states regulate synaptic transmission on Oxt neurons in PVN. The miniature excitatory postsynaptic currents (mEPSCs) onto Oxt neurons in PVN were increased under ad lib fed condition compared to 24 h fasted condition. Furthermore, the NMDA receptor-mediated EPSC on Oxt neurons was increased under fed, compared to fasted, condition. In Oxt neurons, dynein light chain 2 (DYNLL2), a protein suggested to be implicated in the NMDA receptor trafficking to the postsynaptic site, was increased under fed, compared to fasted, condition. The present results suggest that feeding increases excitatory synaptic input on PVN Oxt neurons via mechanisms involving DYNLL2 upregulation and NMDA receptor-mediated synaptic reorganization. Highlights: Fed state facilitates excitatory synaptic transmission on PVN oxytocin neurons. Raised NMDAR underlies facilitation of synaptic transmission on PVN oxytocin neurons. Fed state upregulates NMDAR-transport hub protein, DYNLL2, on PVN oxytocin neurons.