Benzoylsalicylic acid isolated from seed coats of Givotia rottleriformis induces systemic acquired resistance in tobacco and Arabidopsis. (June 2016)

• Record Type: Journal Article
• Authors: Kamatham, Samuel; Neela, Kishore Babu; Pasupulati, Anil Kumar; Pallu, Reddanna; Singh, Surya Satyanarayana; Gudipalli, Padmaja
• Is Part Of: Phytochemistry. Volume 126(2016:Jun.)
• Page Start: 11
• Page End: 22
• Publication Date: 2016-06
• Subjects: Givotia rottleriformis (Euphorbiaceae) -- Benzoylsalicylic acid -- Salicyclic acid -- Acetylsalicylic acid -- Non-expressor of pathogenesis-related gene-1 -- Pathogenesis-related genes -- Systemic acquired resistance -- Hypersensitive response; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2016.03.002
• Languages: English
• ISSNs: 0031-9422

Abstract

Graphical abstract: Highlights: Benzoylsalicylic acid (BzSA) was characterized from the seed coats of Givotia . Salicylic acid pathway intermediates were identified in seed coats of Givotia . BzSA treatment induced the expression of systemic acquired resistance genes. BzSA treatment reduced TMV-induced lesions in tobacco and Arabidopsis. Abstract: Systemic acquired resistance (SAR), a whole plant defense response to a broad spectrum of pathogens, is characterized by a coordinated expression of a large number of defense genes. Plants synthesize a variety of secondary metabolites to protect themselves from the invading microbial pathogens. Several studies have shown that salicylic acid (SA) is a key endogenous component of local and systemic disease resistance in plants. Although SA is a critical signal for SAR, accumulation of endogenous SA levels alone is insufficient to establish SAR. Here, we have identified a new acyl derivative of SA, the benzoylsalicylic acid (BzSA) also known as 2-(benzoyloxy) benzoic acid from the seed coats of Givotia rottleriformis and investigated its role in inducing SAR in tobacco and Arabidopsis. Interestingly, exogenous BzSA treatment induced the expression of NPR1 (Non-expressor of pathogenesis-related gene-1) and pathogenesis related ( PR ) genes. BzSA enhanced the expression of hypersensitivity related ( HSR ), mitogen activated protein kinase ( MAPK ) and WRKY genes in tobacco. Moreover, Arabidopsis NahG plants that were treated with BzSA showed enhanced resistance to tobacco mosaic virus (TMV) as evidenced by reduced leaf necrosis and TMV-coat protein levels in systemic leaves. We, therefore, conclude that BzSA, hitherto unknown natural plant product, is a new SAR inducer in plants.