Blockade of CD47 ameliorates autoimmune inflammation in CNS by suppressing IL-1-triggered infiltration of pathogenic Th17 cells. (May 2016)

• Record Type: Journal Article
• Authors: Gao, Qiangguo; Zhang, Yi; Han, Chaofeng; Hu, Xiang; Zhang, Hua; Xu, Xiongfei; Tian, Jun; Liu, Yiqi; Ding, Yuanyuan; Liu, Juan; Wang, Chunmei; Guo, Zhenhong; Yang, Yongguang; Cao, Xuetao
• Is Part Of: Journal of autoimmunity. Volume 69(2016)
• Page Start: 74
• Page End: 85
• Publication Date: 2016-05
• Subjects: CD47 -- EAE -- Th17 cells -- Cell infiltration -- IL-1β -- iNOS; CNS central nervous system -- DCs dendritic cells -- EAE experimental autoimmune encephalomyelitis -- IL interleukin -- iNOS inducible nitric oxide synthase -- i.p., intraperitoneal i.v., intravenously -- LN lymph node -- MS multiple sclerosis -- NO nitric oxide -- Q-PCR quantitative RT-PCR -- SIRPα signal-regulatory protein alpha -- TSP-1 thrombospondin-1; Autoimmunity -- Periodicals; Autoimmune diseases -- Periodicals; Autoantibodies -- Periodicals; Autoimmune Diseases -- Periodicals; Auto-immunité -- Périodiques; Maladies auto-immunes -- Périodiques; Electronic journals; 616.978005
• DOI: 10.1016/j.jaut.2016.03.002
• Languages: English
• ISSNs: 0896-8411

Abstract

Abstract: The migration of Th17 cells into central nervous system (CNS) tissue is the key pathogenic step in experimental autoimmune encephalomyelitis (EAE) model. However, the mechanism underlying the pathogenic Th17 cell migration remains elusive. Here we report that blockade of CD47 with CD47-Fc fusion protein is effective in preventing and curing EAE by impairing infiltration of Th17 cells into CNS. However, CD47 deficiency does not directly impair the migration of Th17 cells. Mechanistic studies showed that CD47 deficiency inhibited degradation of inducible nitric oxide synthase (iNOS) in proteasome of macrophages by Src activation and led to the increased nitric oxide (NO) production. Then NO suppressed inflammasome activation-induced IL-1β production. This lower IL-1β reduces the expression of IL-1R1 and migration-related chemokine receptors on CD47 −/− Th17 cells, inhibiting the ability of Th17 cells to infiltrate into the CNS of CD47 −/− mice and therefore suppressing EAE development. In vivo administration of exogenous IL-1β indeed promoted the infiltration CD47 −/− Th17 cells into CNS and antagonized the protective role of CD47 deficiency in EAE pathogenesis. Our results demonstrate a potential preventive and therapeutic application of CD47 blockade in controlling EAE development. Highlights: CD47-Fc is effective to control EAE by interrupting the migration of Th17 cells. CD47 blockage decreases IL-1β production by increasing NO expression in macrophages. CD47 promotes degradation of inducible nitric oxide synthase (iNOS) in proteasome. CD47 increases IL-1β production in macrophages through Src tyrosine kinases.