Plant immunity induced by COS-OGA elicitor is a cumulative process that involves salicylic acid. (June 2016)

• Record Type: Journal Article
• Authors: van Aubel, Géraldine; Cambier, Pierre; Dieu, Marc; Van Cutsem, Pierre
• Is Part Of: Plant science. Volume 247(2016:Jun.)
• Page Start: 60
• Page End: 70
• Publication Date: 2016-06
• Subjects: 2D-DIGE two-dimensional difference in-gel electrophoresis -- A.I. active ingredient -- CA2 carbonic anhydrase -- COS chitooligosaccharides -- DAMP damage-associated molecular pattern -- ET ethylene -- Flg22 flagellin22 -- ETI effector-triggered immunity -- GST Glutathione S-transferase -- HR hypersensitive response -- HDS 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase -- HSP heat shock protein -- JA jasmonic acid -- JAZ jasmonate zim repressor -- LSD Fisher's least significant difference -- MEcDP methylerythritolcyclodiphosphate -- NPR1 non expressor of PR genes 1 -- NRQT normalized relative quantities of transcripts -- OGA oligogalacturonides -- PAMP pathogen-associated molecular pattern -- PPO polyphenol oxidase -- PRR pattern recognition receptors -- PTI PAMP-triggered immunity -- RBHR reciprocal best BLAST hit -- ROS reactive oxygen species -- RBOH respiratory burst oxidase homologue -- SA salicylic acid -- SAR systemic acquired resistance -- SNK Student–Newman–Keuls; COS-OGA -- Plant immunity -- Salicylic acid -- SAR -- Subtilisin-like proteases -- Leveillula taurica; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2016.03.005
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Spraying the COS-OGA elicitor on tomato leaves triggers plant innate immunity. COS-OGA spraying induces salicylic acid synthesis in a cumulative way. Plant response to COS-OGA notably involves synthesis of subtilisin-like proteases. COS-OGA efficiently protects tomato plants against powdery mildew in greenhouse. Abstract: Plant innate immunity offers considerable opportunities for plant protection but beside flagellin and chitin, not many molecules and their receptors have been extensively characterized and very few have successfully reached the field. COS-OGA, an elicitor that combines cationic chitosan oligomers (COS) with anionic pectin oligomers (OGA), efficiently protected tomato ( Solanum lycopersicum ) grown in greenhouse against powdery mildew ( Leveillula taurica ). Leaf proteomic analysis of plants sprayed with COS-OGA showed accumulation of Pathogenesis-Related proteins (PR), especially subtilisin-like proteases. qRT-PCR confirmed upregulation of PR-proteins and salicylic acid (SA)-related genes while expression of jasmonic acid/ethylene-associated genes was not modified. SA concentration and class III peroxidase activity were increased in leaves and appeared to be a cumulative process dependent on the number of sprayings with the elicitor. These results suggest a systemic acquired resistance (SAR) mechanism of action of the COS-OGA elicitor and highlight the importance of repeated applications to ensure efficient protection against disease.