A novel long non-coding RNA CYP4B1-PS1-001 regulates proliferation and fibrosis in diabetic nephropathy. (5th May 2016)

• Record Type: Journal Article
• Authors: Wang, Min; Wang, Suyu; Yao, Di; Yan, Qin; Lu, Weiping
• Is Part Of: Molecular and cellular endocrinology. Volume 426(2016)
• Page Start: 136
• Page End: 145
• Publication Date: 2016-05-05
• Subjects: Diabetic nephropathy -- lncRNA -- Proliferation -- Fibrosis; ECM extracellular matrix -- lncRNA long non-coding RNA -- qRT-PCR real-time quantitative PCR -- ELISA enzyme-linked immunosorbent assay -- MMC mouse mesangial cell -- HK-2 human proximal tubular epithelial cell -- BHK-21 baby hamster Syrian kidney cell -- DMEM Dulbecco's modified eagle medium -- FBS fetal bovine serum -- HE hematoxylin-eosin -- PAS periodic acid–Schiff -- GBM glomerular basement membrane -- SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis -- PVDF polyvinylidene difluoride -- PCNA proliferating cell nuclear antigen -- GFP green fluorescent protein -- MIAT myocardial infarction-associated transcript -- PVT1 plasmacytoma variant translocation 1 -- ceRNA competing endogenous RNA -- 450 cytochrome P450 -- ALB albumin -- 20-HETE 20-hidroxyeicosatetraenoic acid -- PPARα peroxisome proliferator-activated receptor alpha -- GO gene ontology -- KEGG Kyoto Encyclopedia of Genes and Genomes -- siRNA small interfering RNA -- TGF-β transforming growth factor-beta1; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2016.02.020
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Diabetic nephropathy is an important microvascular complication of diabetes, and the incidence of end-stage renal disease caused by it are rising annually. Long non-coding RNAs (lncRNAs) are widely regarded to associate with the occurrence and development of various diseases; however, the relationship between lncRNAs and diabetic nephropathy remains largely unknown. This work studied the effect of lncRNAs on diabetic nephropathy pathogenesis. LncRNA microarrays were initially used to detect lncRNAs with altered expression in three cases of kidney tissue from db/db mice with diabetic nephropathy. LncRNAs with differential expression (>2-fold) could be considered candidates. Particularly, CYP4B1-PS1-001 was significantly downregulated in response to early diabetic nephropathy in vitro and in vivo, while overexpression of CYP4B1-PS1-001 inhibited proliferation and fibrosis of mesangial cells. Overall, our data indicate the potential role of CYP4B1-PS1-001 in the proliferation and fibrosis of mice mesangial cells as the prominent features during early stage of diabetic nephropathy, which extend the relationship between lncRNAs and diabetic nephropathy, and may provide a potential therapeutic target and molecular biomarker for the disease. Highlights: 1018 lncRNAs were differentially expressed in kidney tissue from db/db mice with diabetic nephropathy. CYP4B1-PS1-001 was significantly downregulated in response to early diabetic nephropathy. Overexpression of CYP4B1-PS1-001 inhibited proliferation and fibrosis of mesangial cells.