Piscidin: Antimicrobial peptide of rock bream, Oplegnathus fasciatus. (April 2016)

• Record Type: Journal Article
• Authors: Bae, Jin-Sol; Jung, Ji-Min; An, Cheul Min; Kim, Ju-Won; Hwang, Seong Don; Kwon, Mun-Gyeong; Park, Myoung-Ae; Kim, Mu-Chan; Park, Chan-Il
• Is Part Of: Fish & shellfish immunology. Volume 51(2016:Jul.)
• Page Start: 136
• Page End: 142
• Publication Date: 2016-04
• Subjects: Rock bream -- Piscidin -- Antimicrobial peptides -- Antimicrobial activity -- Cytotoxicity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.02.010
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The piscidin family consists of antimicrobial peptides (AMPs) that are mainly found in fish and are crucial effectors of fish innate immune responses. The piscidin family typically has broad-spectrum antimicrobial activity and can modulate immune responses. In this study, we cloned rock bream piscidin (Rbpisc) and investigated its gene expression and biological activity (including antimicrobial and cytotoxic activities). The coding region of Rbpisc consisted of 213 base pairs (bp) encoding 70 amino acid residues. The tertiary structure predicted for Rbpisc includes an amphipathic helix-loop-helix structure. The Rbpisc gene was highly expressed in the gills of healthy fish. The gene expression of Rbpisc increased in the gills after pathogen infection, while the expression was down-regulated in other tissues. A synthetic peptide based on the AMP 12 domain amino acid sequence of Rbpisc appeared to have broad-spectrum antimicrobial activity against various bacteria. However, the synthetic peptide exhibited weak haemolytic activity against fish erythrocytes. These results suggest that Rbpisc might play an important role in the innate immune responses of rock bream. Highlights: We cloned a piscidin from the gill of rock bream. Rbpisc has an amphipathic helix conformation. Rbpisc was induced in the gill of rock bream with and without pathogens infection. Rbpisc exhibited broad antimicrobial activity against various bacteria. Rbpisc induced weak haemolysis against fish erythrocytes.