Comparison of immune response in Pacific white shrimp, Litopenaeus vannamei, after knock down of Toll and IMD gene in vivo. (July 2016)

• Record Type: Journal Article
• Authors: Liu, Yongjie; Song, Lei; Sun, Yuhang; Liu, Tao; Hou, Fujun; Liu, Xiaolin
• Is Part Of: Developmental and comparative immunology. Volume 60(2016)
• Page Start: 41
• Page End: 52
• Publication Date: 2016-07
• Subjects: Litopenaeus vannamei -- Toll pathway -- IMD pathway -- Immune response; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2016.02.004
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: The Toll and immune deficiency (IMD) pathways are essential for inducing immune related genes during invasion of pathogens. In the present study, transcripts of eight pathway-related genes in Litopenaeus vannamei, including Toll, IMD, Pelle, IAP1, TRAF6, ALF, Crustin and Penaeidin3 were analyzed to further understand the potential relationship between Toll and IMD pathway. The high transcription levels of TRAF6, Pelle, Toll, IMD and IAP1 in selected tissues indicates their functional roles in Toll and IMD pathways. The increased mRNA expression of Toll and IMD detected in the early stage might suggest the inducible role of Toll and IMD upon bacterial infection. Moreover, the continuous increase of IMD and the high level of Pelle and TRAF6 in Vibrio anguillarum challenged group indicated that Gram-negative bacterium can activate both the Toll and IMD signaling pathway. Silencing of Toll by a dsRNA-mediated RNAi strongly increased the transcripts of IMD, Pelle, TRAF6, IAP1 and Akirin, knocking down of IMD also markedly increased the transcripts of Toll, Pelle, IAP1 and Akirin. Furthermore, ALF expression was significantly increased in response to V. anguillarum and Micrococcus lysodeikticus challenge, while the transcripts of Crustin and Pen3 in hemocytes were significantly reduced in V. anguillarum group, but rose significantly following M. lysodeikticus infection. In summary, we speculate that Toll and IMD pathway are not independent in shrimp, but linked to defense against bacterial infection. Highlights: Both Gram-positive bacterium and Gram-negative bacterium could induce the Toll pathway. IMD pathway might have important regulatory role in shrimp immunity against Gram-negative bacteria. Toll pathway and IMD pathway are not independent in shrimp, but might be connected by the transcription elements.