Quenching the firefly bioluminescence by various ions. Issue 2 (20th January 2016)

• Record Type: Journal Article
• Authors: Zhang, Huateng; Bai, Haixiu; Jiang, Tianyu; Ma, Zhao; Cheng, Yanna; Zhou, Yubin; Du, Lupei; Li, Minyong
• Is Part Of: Photochemical & photobiological sciences. Volume 15:Issue 2(2016:Feb.)
• Page Start: 244
• Page End: 249
• Publication Date: 2016-01-20
• Subjects: Photochemistry -- Periodicals; Photobiology -- Periodicals; 541.35
• DOI: 10.1039/c5pp00432b
• Languages: English
• ISSNs: 1474-905X

Abstract

Abstract : Some specific ions could selectively inhibit firefly luciferase while having a negligible effect on renilla luciferase, which may be used in the improved dual luciferase reporter gene assay. Abstract : The luciferase reporter gene assay system is broadly applied in various biomedical aspects, including signaling pathway dissection, transcriptional activity analysis, and genetic toxicity testing. It significantly improves the experimental accuracy and reduces the experimental error by the addition of an internal control. In the current research, we discovered some specific ions that could selectively inhibit firefly luciferase while having a negligible effect on renilla luciferase in vitro in the dual-reporter gene assay. We showed that these ionic compounds had a high potential of being utilized as quench-and-activate reagents in the dual-reporter assay. Furthermore, results from kinetic studies on ion-mediated quenching effects indicated that different ions have distinct inhibition modes. Our study is anticipated to guide a more affordable design of quench-and-activate reagents in biomedicine and pharmaceutical analysis.