Human IgG detection in serum on polymer based Mach‐Zehnder interferometric biosensors. Issue 3 (10th December 2015)

• Record Type: Journal Article
• Authors: Melnik, Eva; Bruck, Roman; Müellner, Paul; Schlederer, Thomas; Hainberger, Rainer; Lämmerhofer, Michael
• Is Part Of: Journal of biophotonics. Volume 9:Issue 3(2016)
• Page Start: 218
• Page End: 223
• Publication Date: 2015-12-10
• Subjects: Mach‐Zehnder interferometer -- polymer waveguide -- biosensor -- human IgG -- polyimide -- human serum -- injection molding; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201500280
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : We report a new method for detecting human IgG (hIgG) in serum on integrated‐optical Mach‐Zehnder interferometer biosensors realized in a high index contrast polymer material system. In the linear range of the sensor (5–200 nM) we observed excellent signal recoveries (95–110%) in buffer and serum samples, which indicate the absence of matrix effects. Signal enhancement was reached by using secondary anti‐human IgG antibodies, which bind to immobilized target IgGs and allow detecting concentrations down to 100 pM. This polymer based optical sensor is fully compatible with cost‐efficient mass production technologies, which makes it an attractive alternative to inorganic optical sensors.Graphical abstract of the hIgG measured on polymer based photonic sensors using a direct binding assay and a signal enhancement strategy with secondary antibodies. Abstract : A new method is reported for detecting human IgG (hIgG) in serum on integrated‐optical Mach‐Zehnder interferometer biosensors realized in a high index contrast polymer material system. In the linear range of the sensor excellent signal recoveries in buffer and serum samples were observed, which indicates the absence of matrix effects. Thise polymer‐based optical sensor is fully compatible with cost‐efficient mass production technologies which makes it an attractive alternative to inorganic optical sensors.