Enzymatic hydrolysis of chitin pretreated by rapid depressurization from supercritical 1, 1, 1, 2-tetrafluoroethane toward highly acetylated oligosaccharides. (June 2016)

• Record Type: Journal Article
• Authors: Villa-Lerma, Guadalupe; González-Márquez, Humberto; Gimeno, Miquel; Trombotto, Stéphane; David, Laurent; Ifuku, Shinsuke; Shirai, Keiko
• Is Part Of: Bioresource technology. Volume 209(2016)
• Page Start: 180
• Page End: 186
• Publication Date: 2016-06
• Subjects: Supercritical 1, 1, 1, 2-tetrafluoroethane -- scR134a -- Chitin -- Lecanicillium lecanii -- Chitinases; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.02.138
• Languages: English
• ISSNs: 0960-8524

Abstract

Abstract : Graphical abstract: Highlights: Chitinases production from fungus Lecanicillium lecanii was optimized in SCF. Rapid expansion of scR134a and fibrillation lowered I CR but kept high acetylation. The process provides small particle size to oligosaccharides with high DP and F A . This novel process achieves high product yields. Abstract: The hydrolysis of chitin treated under supercritical conditions was successfully carried out using chitinases obtained by an optimized fermentation of the fungus Lecanicillium lecanii . The biopolymer was subjected to a pretreatment based on suspension in supercritical 1, 1, 1, 2-tetrafluoroethane (scR134a), which possesses a critical temperature and pressure of 101 °C and 40 bar, respectively, followed by rapid depressurization to atmospheric pressure and further fibrillation. This methodology was compared to control untreated chitins and chitin subjected to steam explosion showing improved production of reducing sugars (0.18 mg/mL), enzymatic hydrolysis and high acetylation ( F A of 0.45) in products with degrees of polymerization between 2 and 5.