Calcium signaling in human pluripotent stem cells. Issue 2 (March 2016)

• Record Type: Journal Article
• Authors: Apáti, Ágota; Berecz, Tünde; Sarkadi, Balázs
• Is Part Of: Cell calcium. Volume 59:Issue 2/3(2016)
• Page Start: 117
• Page End: 123
• Publication Date: 2016-03
• Subjects: hPSC human pluripotent stem cell -- mPSC mouse pluripotent stem cell -- hESC human embryonic stem cell -- iPSC induced pluripotent stem cell -- GECI genetically engineered calcium indicator -- EGF epidermal growth factor -- FGF fibroblast growth factor -- FGFR fibroblast growth factor receptor -- IGF insulin-like growth factor -- TGFβ ransforming growth factor β -- Wnt Wingless/Integrated -- GPCR G protein-coupled receptor -- ERK extracellular signal-regulated kinase -- PI3K phosphoinositide 3-kinase -- MEK MAPK/ERK kinase -- MAPK mitogen-activated protein kinase -- PLC-γ phospholipase C-γ -- mTOR mammalian target of rapamycin -- FZ frizzled -- DVL disheveled -- PKC protein kinase C -- GSK3 glycogen synthase kinase 3 -- LPA lysophosphatidic acid -- SERCA sarcoplasmic/endoplasmic calcium ATPase -- CPA cyclopiazonic acid -- TG thapsigargin -- CAMKII calcium/calmodulin-dependent protein kinase II -- AP alkaline phosphatase -- GABA γ-aminobutyric acid -- LIF leukemia inhibitory factor -- JAK Janus kinase -- STAT signal transducer and activator of transcription -- BMP bone morphogenetic protein -- HERVH human endogenous retrovirus H; Calcium signals -- Pluripotent stem cells -- Pluripotency maintenance -- Naïve and primed state -- Calcium sensitive dyes -- Genetically engineered calcium indicators; Calcium -- Metabolism -- Periodicals; Vertebrates -- Physiology -- Periodicals; Calcium -- Physiological effect -- Periodicals; Cell physiology -- Periodicals; Calcium in the body -- Periodicals; 572.516
• DOI: 10.1016/j.ceca.2016.01.005
• Languages: English
• ISSNs: 0143-4160

Abstract

Graphical abstract: Highlights: Methods for studying calcium signals in human pluripotent stem cells is reviewed. An overview of calcium signaling involved in pluripotency maintenance is provided. Various forms of human pluripotent cells and the relevant signaling is discussed. Abstract: Human pluripotent stem cells provide new tools for developmental and pharmacological studies as well as for regenerative medicine applications. Calcium homeostasis and ligand-dependent calcium signaling are key components of major cellular responses, including cell proliferation, differentiation or apoptosis. Interestingly, these phenomena have not been characterized in detail as yet in pluripotent human cell sates. Here we review the methods applicable for studying both short- and long-term calcium responses, focusing on the expression of fluorescent calcium indicator proteins and imaging methods as applied in pluripotent human stem cells. We discuss the potential regulatory pathways involving calcium responses in hPS cells and compare these to the implicated pathways in mouse PS cells. A recent development in the stem cell field is the recognition of so called "naïve" states, resembling the earliest potential forms of stem cells during development, as well as the "fuzzy" stem cells, which may be alternative forms of pluripotent cell types, therefore we also discuss the potential role of calcium homeostasis in these PS cell types.